Apparatus and method for detecting nucleic acid amplification products
First Claim
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1. An apparatus comprising:
- a sample holder for holding a sealed reaction chamber, the sealed reaction chamber including a sample, an optical interface and an air gap between the sample and the optical interface;
a fiber optic cable positioned outside the sealed reaction chamber to deliver an excitation beam to the sample housed within the reaction chamber and to receive light emitted by the sample; and
a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber to focus the excitation beam through the optical interface and the air gap into a volume of the sample and to collect and transmit to the fiber optic cable through the optical interface and the air gap light emitted within the volume of the sample.
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Abstract
An apparatus is provided which includes a sample holder for holding a reaction chamber which includes an optical interface, a fiber optic cable for delivering an excitation beam to a sample housed within the reaction chamber and for receiving light emitted by the sample, and a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber for focusing the excitation beam through the optical interface and within a volume of the sample and for collecting and transmitting to the fiber optic cable light emitted within the volume of the sample.
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Citations
32 Claims
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1. An apparatus comprising:
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a sample holder for holding a sealed reaction chamber, the sealed reaction chamber including a sample, an optical interface and an air gap between the sample and the optical interface; a fiber optic cable positioned outside the sealed reaction chamber to deliver an excitation beam to the sample housed within the reaction chamber and to receive light emitted by the sample; and a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber to focus the excitation beam through the optical interface and the air gap into a volume of the sample and to collect and transmit to the fiber optic cable through the optical interface and the air gap light emitted within the volume of the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An apparatus comprising:
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a sealable reaction chamber which includes an optical interface; a fiber optic cable positioned outside the sealable reaction chamber to deliver an excitation beam to a sample housed within the reaction chamber and to receive light emitted by the sample; and a lens co-axially disposed with the fiber optic cable and positioned outside the reaction chamber to focus the excitation beam through the optical interface into a volume of the sample and to collect and transmit to the fiber optic cable through the optical interface light emitted within the volume of the sample. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. An apparatus comprising:
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a plurality of sample holders for holding a plurality of sealed reaction chambers, each sealed reaction chamber including a sample, an optical interface and an air gap between the sample and the optical interface; a plurality of fiber optic cables positioned outside the sealed reaction chambers to deliver an excitation beam to samples housed within the plurality of reaction chambers and to receive light emitted by the samples; and a plurality of lenses co-axially disposed with the fiber optic cables and positioned outside the reaction chambers to focus the excitation beams through the optical interfaces and the air gaps into volumes of the samples and to collect and transmit to the fiber optic cables through the optical interface and the air gap light emitted within the volumes of the samples. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. An apparatus comprising:
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a plurality of sealable reaction chambers which each include an optical interface; a plurality of fiber optic cables positioned outside the sealable reaction chambers to deliver excitation beams to samples housed within the plurality of reaction chambers and to receive light emitted by the samples; and a plurality of lenses co-axially disposed with the fiber optic cables and positioned outside the reaction chambers to focus the excitation beams through the optical interfaces into volumes of samples housed within the plurality of reaction chambers and to collect and transmit to the fiber optic cables through the optical interfaces light emitted within the volumes of the samples. - View Dependent Claims (24, 25, 26, 27)
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28. A method for monitoring the formation of a nucleic acid amplification reaction product comprising:
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amplifying a nucleic acid sequence in a sample in a sealed reaction chamber which includes an optical interface and an air gap between the sample and the optical interface; delivering an excitation beam from a fiber optic cable positioned outside the sealed reaction chamber into a volume of the sample through the optical interface and the air gap; and collecting light emitted by the volume of sample within the fiber optic cable through the optical interface and the air gap. - View Dependent Claims (29, 30, 31, 32)
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Specification