Oligonucleotide modulation of cell adhesion
First Claim
1. An antisense oligonucleotide targeted to a translation initiation site of a nucleic acid presented in FIG. 1 encoding intercellular adhesion molecule-1, said antisense oligonucleotide comprising from 8 to 25 nucleotide subunits, wherein said antisense oligonucleotide modulates the production of said intercellular adhesion molecule-1.
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Abstract
Compositions and methods are provided for the treatment and diagnosis of diseases amenable to treatment through modulation of the synthesis or metabolism of intercellular adhesion molecules. In accordance with preferred embodiments, oligonucleotides are provided which are specifically hybridizable with nucleic acids encoding intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial leukocyte adhesion molecule-1. The oligonucleotide comprises nucleotide units sufficient in identity and number to effect said specific hybridization. In other preferred embodiments, the oligonucleotides are specifically hybridizable with a transcription initiation site, a translation initiation site, 5'"'"'-untranslated sequences, 3'"'"'-untranslated sequences, and intervening sequences. Methods of treating animals suffering from disease amenable to therapeutic intervention by modulating cell adhesion proteins with an oligonucleotide specifically hybridizable with RNA or DNA corresponding to one of the foregoing proteins are disclosed. Methods for treatment of diseases responding to modulation cell adhesion molecules are disclosed.
23 Citations
4 Claims
- 1. An antisense oligonucleotide targeted to a translation initiation site of a nucleic acid presented in FIG. 1 encoding intercellular adhesion molecule-1, said antisense oligonucleotide comprising from 8 to 25 nucleotide subunits, wherein said antisense oligonucleotide modulates the production of said intercellular adhesion molecule-1.
- 4. An antisense oligonucleotide up to 50 nucleic acid base units in length and comprising the sequence
- space="preserve" listing-type="tabular">______________________________________ 5'"'"'-CGGGGGCTGCTGGGAGCCAT SEQ ID NO;
8, 5'"'"'-TGGGAGCCATAGCGAGGC SEQ ID NO;
1, 5'"'"'-TGGGAGCCATAGCGAG SEQ ID NO;
20, 5'"'"'-GGAGCCATAGCGAGGC SEQ ID NO;
21, 5'"'"'-AGCCATAGCGAGGC SEQ ID NO;
17, 5'"'"'-CCATAGCGAGGC SEQ ID NO;
18, 5'"'"'-CATAGCGAGGCTGAGGTTGC SEQ ID NO;
7, or 5'"'"'-ATAGCGAGGC SEQ ID NO;
19. ______________________________________
- space="preserve" listing-type="tabular">______________________________________ 5'"'"'-CGGGGGCTGCTGGGAGCCAT SEQ ID NO;
Specification