Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate

• US 6,017,702 A
• Filed: 01/21/1997
• Issued: 01/25/2000
• Est. Priority Date: 12/05/1996
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Perkinelmer Incorporated
• Inventors: Lee, Linda G., Rosenblum, Barnett B.
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): SHOEMAKER, DEBRA GUNNERSEN
• Application Number:
  US08/785,581
• Time in Patent Office: 1,099 Days
• Field of Search: 435/6, 435/91.2, 435/91.5, 435/91, 435/810, 536/24.33, 935/8, 935/78, 436/800
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

A chain-termination type DNA sequencing method is disclosed wherein deoxynucleotide 2'"'"'-deoxythymidine-5'"'"'-triphosphate is replaced by 2'"'"'-deoxyuridine-5'"'"'-triphosphate, or analogs thereof Kits for performing the method are also provided.

Claims