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Solid phase amplification process

  • US 6,017,738 A
  • Filed: 12/09/1996
  • Issued: 01/25/2000
  • Est. Priority Date: 11/01/1991
  • Status: Expired due to Fees
First Claim
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1. A method for detecting a target nucleic acid sequence by a single-stage amplification process conduct ed within a single container, said method comprising:

  • (a) conducting, within a single container, an amplification process having only a single stage comprising the following steps;

    (i) providing a first primer hybridizing to the target nucleic acid sequence, wherein the first primer is immobilized on an immobile solid phase support by a direct chemical linkage between the first primer and the solid phase support, wherein the solid phase support forms a part of or is insertable into a container for a sample to be tested,(ii) providing a second primer hybridizing to the target nucleic acid sequence in the opposite direction, wherein the second primer is labeled with a detectable label,(iii) adding an aliquot of the first primer or other primer(s) to a sample solution containing nucleic acid sequences, in the container together with said first primer of step (i) and said second primer, and(iv) reacting the first, second and other primer(s) with the sample containing nucleic acid sequences under conditions which allow amplification of the nucleic acid sequences that hybridize to the first, second and other primers in the container for the sample, and(b) within the same container, detecting the presence of bound second primer.

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