Periodate oxidative method for attachment and crosslinking of biomolecules to medical device surfaces
First Claim
1. A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the method comprising the steps of:
- (a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising an amine moiety;
(b) providing two or more biomolecules, the biomolecules comprising amine moieties and 2-aminoalcohol moieties, combining a periodate with the biomolecules to oxidize the 2-aminoalcohol moieties to form aldehyde moieties;
(c) combining the biomolecules with the surface;
(d) allowing the aldehyde moieties to combine with the amine moieties of the biomolecules and the amine moiety of the biomaterial to form imine moieties; and
(e) reacting the imine moieties with a reducing agent to form amine linkages, the amine linkages immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating.
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Accused Products
Abstract
Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A 2-aminoalcohol moiety of a biomolecule is oxidized with a periodate to an aldehyde moiety which is reacted with an amine moiety on the surface of a medical device to form an imine moiety, and the imine moiety is reduced to form an amine linkage immobilizing a coating of the biomolecule on the surface. Conversely, the biomolecule can contain the amine moiety and the 2-aminoalcohol moiety can be on the surface. In another method, a biomolecule coating containing an amine moiety and a 2-aminoalcohol moiety is immobilized on the surface of a medical device, and the 2-aminoalcohol moiety is oxidized with a periodate to an aldehyde moiety which reacts with the amine moiety to crosslink the coating. A crosslinked biomaterial may be formed by combining a periodate with two or more biomolecules containing amine moieties and 2-aminoalcohol moieties to oxidize the 2-aminoalcohol moieties to aldehyde moieties, and allowing the aldehyde moieties to react with the amine moieties. When a medical device surface containing amine moieties is present with the two or more biomolecules, some of the aldehyde moieties react with the amine moieties on the medical device surface resulting in both immobilizing and crosslinking of the biomolecules on the surface.
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Citations
19 Claims
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1. A method of forming a crosslinked coating on a surface of a medical device, the coating imparting improved biocompatibility characteristics to the surface, the method comprising the steps of:
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(a) providing the medical device, the device having a suitable biomaterial forming the surface, the biomaterial comprising an amine moiety; (b) providing two or more biomolecules, the biomolecules comprising amine moieties and 2-aminoalcohol moieties, combining a periodate with the biomolecules to oxidize the 2-aminoalcohol moieties to form aldehyde moieties; (c) combining the biomolecules with the surface; (d) allowing the aldehyde moieties to combine with the amine moieties of the biomolecules and the amine moiety of the biomaterial to form imine moieties; and (e) reacting the imine moieties with a reducing agent to form amine linkages, the amine linkages immobilizing and crosslinking the biomolecules on the surface, the immobilized and crosslinked biomolecules forming the coating. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification