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Methods for the detection of loss of heterozygosity

  • US 6,020,137 A
  • Filed: 11/23/1998
  • Issued: 02/01/2000
  • Est. Priority Date: 08/14/1996
  • Status: Expired due to Term
First Claim
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1. A method for detecting the presence of a mutant DNA in a pooled biological sample, comprising the steps of:

  • a) pooling biological samples collected from a plurality of members of a patient population;

    b) determining from said pooled samples a number X of a first wild-type polynucleotide characteristic of a genomic region of said members of said patient population that is not mutated in said subpopulation of transformed cells;

    c) determining from said pooled biological sample a number Y of a second wild-type polynucleotide in a genomic region of one or more members of said patient population suspected of being mutated in said subpopulation of transformed cells; and

    d) determining whether a difference exists between number X and Y, the presence of a statistically-significant difference being indicative of a clonal subpopulation of transformed cells in said pooled biological sample.

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