Phycobiliprotein-linker peptide complex fluorescent tracer and methods of using the same
First Claim
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1. A fluorescent method of detecting and/or determining an analyte in a medium in which it may be present, characterised in that a phycobiliprotcin-linker peptide complex is used as a fluorescent tracer.
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Abstract
The use of a phycobiliprotein-linker peptide complex as a fluorescent tracer in a fluorescent method of detecting and/or determining an analyte in a medium in which it may be present, is disclosed. Fluorescent conjugates consisting of said complex covalently bonded to one of the elements of a ligand/receptor specific binding pair, are also disclosed.
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29 Claims
- 1. A fluorescent method of detecting and/or determining an analyte in a medium in which it may be present, characterised in that a phycobiliprotcin-linker peptide complex is used as a fluorescent tracer.
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6. A homogenous fluorescent method of detecting and/or determining an analyte in a medium in which it may be present, by displaying the product of the reaction between the analyte and at least one corresponding receptor, consisting of:
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1) adding to said medium a first reagent constituted of at least one receptor of said analyte, 2) adding a second reagent selected from the analyte or at least one of its receptors, one of said first reagent or said second reagent coupled with a flourescent donor compound constituted by a rare earth cryptate, chelate or macrocyclic complex and other reagent being coupled with a fluorescent acceptor compound, it being possible for the order of addition of the reagents to be the reverse order and, after excitation of the mixture by a source of light at the excitation wavelength of the fluorescent donor compound, 3) measuring the emission signal of the fluorescent acceptor compould, and 4) correlating the measured emission signal of the fluorescent acceptor compound to the presence and/or amount of analyte, characterised in that a phycobiliprotein-linker peptide complex is used as a fluorescent acceptor compound. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 24, 25, 26, 27)
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- 16. A fluorescent conjugate constituted of a phycobiliprotein-linker peptide complex covalently bound to one of the elements of a ligand/receptor specific binding pair.
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