Plant chlorophyll content meter

• US 6,020,587 A
• Filed: 01/06/1998
• Issued: 02/01/2000
• Est. Priority Date: 01/06/1998
• Status: Expired due to Term

• Current Assignee: United States Department Of Health And Human Services (Government of the United States of America)
• Original Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Inventors: Spiering, Bruce A., Carter, Gregory A.
• Primary Examiner(s): EVANS, FANNIE L
• Application Number:
  US09/003,212
• Time in Patent Office: 756 Days
• Field of Search: 356/402, 356/416, 356/419, 250/339.11
• US Class Current: 250/339.11
• CPC Class Codes: G01N 2021/8466 Investigation of vegetal material, e.g. leaves, plants, fruits
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427

Abstract

A plant chlorophyll content meter is described which collects light reflected from a target plant and separates the collected light into two different wavelength bands. These wavelength bands, or channels, are described as having center wavelengths of 700 nm and 840 nm. The light collected in these two channels are processed using photo detectors and amplifiers. An analog to digital converter is described which provides a digital representation of the level of light collected by the lens and falling within the two channels. A controller provided in the meter device compares the level of light reflected from a target plant with a level of light detected from a light source, such as light reflected by a target having 100% reflectance, or transmitted through a diffusion receptor. The percent of reflection in the two separate wavelength bands from a target plant are compared to provide a ratio which indicates a relative level of plant physiological stress. A method of compensating for electronic drift is described where a sample is taken when a collection lens is covered to prevent light from entering the device. This compensation method allows for a more accurate reading by reducing error contributions due to electronic drift from environmental conditions at the location where a hand-held unit is used.

Claims

1. A chlorophyll content meter comprising:

a first optical bandpass filter for filtering light received through an optical lens, the first bandpass filter having a central wavelength of transmission of 700 nm;

a second optical bandpass filter for filtering light received through an optical lens, the second bandpass filter having a central wavelength of transmission of 840 nm;

detector circuitry for detecting a level of light output from each of the first and second optical bandpass filters and converting the detected levels into digital representations; and

a controller for receiving the digital representations and calculating a relative chlorophyll content of a target plant, whereby the controller calculates a percent of light reflected from the target plant by comparing outputs from the first and second optical bandpass filter produced from light reflected from the target plant against outputs from the first and second optical bandpass filter produced from a light source.

2. The chlorophyll content meter of claim 1 further comprising an alphanumeric display for providing visual output indicating a content level of chlorophyll of the target plant.

3. The chlorophyll content meter of claim 1 wherein the controller calculates a ratio of the percent reflectance of the target plant as output by the first optical bandpass filter to the percent reflectance of the target plant as output by the second optical bandpass filter.

4. The chlorophyll content meter of claim 1 wherein the detector circuitry comprises:

a first detector circuit for detecting light output from the first optical bandpass filter;

a second detector circuit for detecting light output from the second optical bandpass filter;

a first amplifier circuit for amplifying an output from the first detector circuit;

a second amplifier circuit for amplifying an output from the second detector circuit; and

an analog to digital converter circuit for converting outputs from the first and second amplifier circuits to digital data.

5. The chlorophyll content meter of claim 4 wherein the detector circuitry further comprises a multiplex circuit for selectively coupling outputs from the first and second amplifier circuits with the analog to digital converter.

6. The chlorophyll content meter of claim 1 further comprising a memory circuit coupled to the controller for storing data representing output from the first and second bandpass filters produced from light reflected from a reference target for use by the controller.

7. The chlorophyll content meter of claim 1 wherein light from the light source is collected from a reflective target to produce the outputs from the first and second optical bandpass filters.

8. A hand-held light reflectance meter for use in measuring plant chlorophyll content comprising:

an optical lens for collecting light from a light source or reflected from a target plant under test;

an optical beam splitter for dividing light collected by the optical lens into first and second light beams;

a first optical bandpass filter for receiving the first light beam from the optical beam splitter and providing a light output having a center wavelength of approximately 700 nm;

a first light detector coupled to the first optical bandpass filter for providing an output indicating a level of the light output from the first optical bandpass filter;

a second optical bandpass filter for receiving the second a light beam from the optical beam splitter and providing a light output having a center wavelength of approximately 840 nm;

a second light detector coupled to the second optical bandpass filter for providing an output indicating a level of the light output from the second optical bandpass filter;

processing circuitry for converting the outputs from the first and second light detectors into a digital data representation;

a controller coupled to the processing circuitry for calculating plant chlorophyll content levels using digital data representing light from the light source and digital data representing light reflected from the target plant, the controller providing an output of light reflected from the target plant as a percent of the light from the light source, and the controller providing an output of the ratio of the percent of reflected light output by the first optical bandpass filter to the percent of reflected light output by the second optical bandpass filter; and

a display for providing a user with the output from the controller.

9. The hand-held light reflectance meter of claim 8 further comprising a memory for storing data representing light reflected from a reference target.

10. The hand-held light reflectance meter of claim 9 wherein the memory further stores data representing an offset value for use in compensating for variables due to electronic drift resulting from thermal variations.

11. The hand-held light reflectance meter of claim 8 wherein the controller compensates for variables due to electronic drift resulting from thermal variations.

12. The hand-held light reflectance meter of claim 8 further comprising input controls for controlling operation of the reflectance meter.

13. The hand-held light reflectance meter of claim 8 wherein the processing circuitry comprises a multiplex circuit for selectively coupling the outputs from the first and second light detectors to the controller.

14. The hand-held light reflectance meter of claim 8 wherein the first and second optical bandpass filters each have a bandwidth of 10 nm.

15. The hand-held light reflectance meter of claim 8 wherein light from the light source is collected from a reflective target.

16. A method of detecting plant chlorophyll content for identifying early plant stress using a chlorophyll content meter, the method comprising:

detecting light from a light source having a first wavelength of 700 nm using the chlorophyll content meter;

detecting light from the light source having a second wavelength of 840 nm using the chlorophyll content meter;

detecting light reflected from a target plant having a first wavelength using the chlorophyll content meter;

detecting light reflected from the target plant having a second wavelength using the chlorophyll content meter;

calculating a first reflected percent of light reflected from the target plant having the first wavelength from the light detected from the light source having the first wavelength;

calculating a second reflected percent of light reflected from the target plant having the second wavelength from the light detected from the light source having the second wavelength;

calculating a ratio of the first reflected percent to the second reflected percent; and

providing an output indicating a relative plant stress level based upon the calculated ratio.

17. The method of claim 16 wherein each of the steps of detecting light comprises:

collecting reflected light using an optical lens;

filtering the collected light using an optical bandpass filter having a pre-selected bandwidth;

detecting an output level of light passing through the optical bandpass filter; and

converting the detected output level into a digital data value.

18. The method of claim 16 wherein a low ratio indicates a low level of plant stress, and a high ratio indicates a high level of plant stress.

19. The method of claim 16 wherein the light detected from the light source is reflected from a target which substantially reflects 100 percent of light provided by a light source.

20. A chlorophyll content meter comprising:

a first optical bandpass filter for filtering light received through an optical lens, the first bandpass filter having a central wavelength of transmission of 700 nm;

a second optical bandpass filter for filtering light received through an optical lens, the second bandpass filter having a central wavelength of transmission;

detector circuitry for detecting a level of light output from each of the first and second optical bandpass filters and converting the detected levels into digital representations; and

a controller for receiving the digital representations and calculating a relative chlorophyll content of a target plant, whereby the controller calculates a percent of light reflected from the target plant by comparing outputs from the first and second optical bandpass filter produced from light reflected from the target plant against outputs from the first and second optical bandpass filter produced from a light source, wherein the controller calculates a ratio of the percent reflectance of the target plant as output by the first optical bandpass filter to the percent reflectance of the target plant as output by the second optical bandpass filter.

21. The chlorophyll content meter of claim 20 wherein the central wavelength of transmission of the second optical bandpass filter is 840±

5 nm.

22. The chlorophyll content meter of claim 20 further comprising an alphanumeric display for providing visual output indicating a content level of chlorophyll of the target plant.

23. The chlorophyll content meter of claim 20 wherein the detector circuitry comprises:

a first detector circuit for detecting light output from the first optical bandpass filter;

a second detector circuit for detecting light output from the second optical bandpass filter;

a first amplifier circuit for amplifying an output from the first detector circuit;

a second amplifier circuit for amplifying an output from the second detector circuit; and

an analog to digital converter circuit for converting outputs from the first and second amplifier circuits to digital data.

24. A method of detecting plant chlorophyll content for identifying early plant stress using a chlorophyll content meter, the method comprising:

detecting light from a light source having a first wavelength of 700 nm using the chlorophyll content meter;

detecting light from the light source having a second wavelength using the chlorophyll content meter;

detecting light reflected from a target plant having a first wavelength using the chlorophyll content meter;

detecting light reflected from the target plant having a second wavelength using the chlorophyll content meter;

calculating a first reflected percent of light reflected from the target plant having the first wavelength from the light detected from the light source having the first wavelength;

calculating a second reflected percent of light reflected from the target plant having the second wavelength from the light detected from the light source having the second wavelength;

calculating a ratio of the first reflected percent to the second reflected percent; and

providing an output indicating a relative plant stress level based upon the calculated ratio.

25. The method of claim 24 detecting light comprises:

collecting reflected light using an optical lens;

filtering the collected light using an optical bandpass filter having a pre-selected bandwidth;

detecting an output level of light passing through the optical bandpass filter; and

converting the detected output level into a digital data value.

26. The method of claim 24 wherein a low ratio indicates a low level of plant stress, and a high ratio indicates a high level of plant stress.

27. The method of claim 24 wherein the light detected from the light source is reflected from a target substantially reflects 100 percent of light provided by a light source.

28. The method of claim 24 wherein the second wavelength is 840±

5 nm.