Sol-gel matrices for direct colorimetric detection of analytes

• US 6,022,748 A
• Filed: 08/29/1997
• Issued: 02/08/2000
• Est. Priority Date: 08/29/1997
• Status: Expired due to Fees

• Current Assignee: Sandia Corporation (Martin Marietta Corporation), Regents of the University of California (University of California)
• Original Assignee: Sandia Corporation - New Mexico Regents of The University of California
• Inventors: Charych, Deborah H., Sasaki, Darryl, Yamanaka, Stacey
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US08/920,501
• Time in Patent Office: 893 Days
• Field of Search: 436/528, 436/829, 436/527, 436/535, 436/811, 436/815, 436/805, 436/823, 424/1.21, 424/450, 424/94.3, 424/812
• US Class Current: 436/527
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C03C 14/008 the non-glass component being in molecular form
• CPC Class Codes: C03C 4/0007 for biologically-compatible glass
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/823 Immunogenic carrier or carrier per se
• CPC Class Codes: Y10S 436/829 Liposomes, e.g. encapsulation

Abstract

The present invention relates to methods and compositions for the direct detection of analytes using color changes that occur in immobilized biopolymeric material in response to selective binding of analytes to their surface. In particular, the present invention provides methods and compositions related to the encapsulation of biopolymeric material into metal oxide glass using the sol-gel method.

Claims

1. A composition comprising colorimetric diacetylene polymers encapsulated in porous sol-gel glass, wherein said colorimetric diacetylene polymers comprise one or more ligands that are capable of binding to one or more analytes, and wherein said colorimetric diacetylene polymers are capable of changing color upon the binding of said one or more analytes to said one or more ligands.

2. The composition of claim 1, wherein said sol-gel glass comprises tetramethylorthosilicate.

3. The composition of claim 1, wherein said calorimetric diacetylene polymers have a structure selected from the group consisting of liposomes, films, multilayers, braided, lamellar, helical, tubular, and fiber-like shapes, solvated rods, solvated coils, and combinations thereof.

4. The composition of claim 1, wherein said colorimetric diacetylene polymers comprise polymerized lipid monomers selected from the group consisting of 5,7-docosadiynoic acid, 10,12-pentacosadiynoic acid, 5,7-pentacosadiynoic acid, and combinations thereof.

5. The composition of claim 4, wherein said polymerized lipid monomers contain head groups selected from the group consisting of carboxylic acid, hydroxyl groups, amine groups, amino acid derivatives, and hydrophobic groups.

6. The composition of claim 1, wherein said ligands are selected from the group consisting of peptides, carbohydrates, nucleic acids, biotin, drugs, chromophores, antigens, chelating compounds, molecular recognition complexes, ionic groups, polymerizable groups, linker groups, electron donors, electron acceptor groups, hydrophobic groups, hydrophilic groups, receptor binding groups, antibodies, and combinations thereof.