Integrated fluorescence-based biochemical sensor

• US 6,024,923 A
• Filed: 05/17/1999
• Issued: 02/15/2000
• Est. Priority Date: 10/01/1996
• Status: Expired due to Term

• Current Assignee: Texas Instruments, Inc.
• Original Assignee: Texas Instruments, Inc.
• Inventors: Arbuthnot, Diane, Melendez, Jose, Carr, Richard A.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/313,466
• Time in Patent Office: 274 Days
• Field of Search: 422/82.05, 422/82.08, 422/82.11, 422/91, 436/68, 436/136, 436/138, 436/165, 436/172
• US Class Current: 422/82.08
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 2201/022 Casings

Abstract

An integrated biochemical sensor (200) for detecting the presence of one or more specific samples (240) having a device platform (355) with a light absorbing upper surface and input/output pins (375) is disclosed. An encapsulating housing (357) provides an optical transmissive enclosure which covers the platform (355) and has a layer of fluorescence chemistry on its outer surface (360). The fluorophore is chosen for its molecular properties in the presence of the sample analyte (240). The detector (370), light sources (365, 367, 407, 409) are all coupled to the platform (355) and encapsulated within the housing (357). A filter (375) element is used to block out unwanted light and increase the detector'"'"'s (370) ability to resolve wanted emission light.

Claims

1. A sensor assembly comprising:

a platform having an upper surface and a bottom surface;

a light source coupled to the upper surface of the platform;

a detector coupled to the upper surface of the platform adjacent the light source; and

an encapsulating housing extending over the platform and arranged to receive light from the light source;

where at least a portion of the exterior surface of the housing has a fluorescence emitting chemistry layer disposed upon it to form a sensing surface, the fluorescence emitting chemistry layer has a characteristic quality that interacts with a specific analyte sample to produce a change in the layer'"'"'s fluorescence emission;

where the light source emits light having an wavelength that excites the fluorescence emitting chemistry layer; and

where the housing has a substantially dome-shaped portion, the dome-shaped portion having a location and a radius such that the normal direction of the surface of the dome-shaped portion is oriented towards the detector, thereby increasing the amount of light emitted from the fluorescence emitting chemistry layer that impinges upon the detector.

2. An integrated miniaturized sensor for indicating the presence of a given analyte sample comprising:

a first light source;

a sensing surface spatially arranged about the first light source to receive light;

a detector mounted proximate the first light source and positioned to intercept light

emitted from the sensing surface; and

a light transmissive housing encapsulating the first light source and the detector;

wherein, the exterior of the housing includes the sensing surface, and the housing is shaped to reflect light from the first light source away from the detector and toward an optical sink.

3. The integrated miniaturized sensor according to claim 2, wherein the sensing surface portion of the housing is substantially dome-shaped, the dome-shaped portion of the housing approximately centered on the detector.

4. The sensor according to claim 3, further including second, third and fourth light sources spatially arranged with respect to the first light source and each other to uniformly distribute light about the housing.

5. The integrated miniaturized sensor according to claim 2, further comprising a platform with an upper surface and a bottom surface, the upper surface coupled to the first light source, the sensing surface, the detector and the optical sink, the upper surface being coated with a light absorbing material.

6. The sensor according to claim 5, further comprising an interface coupled to the bottom surface of the platform.

7. A fluorescence-based sensor for selectively identifying the presence of oxygen comprising:

a platform having an upper and a bottom surface;

a blue light emitting diode coupled to the upper surface of the platform;

a photodiode coupled to the upper surface of the platform adjacent the diode; and

an encapsulating housing extending over the upper surface of the platform, the housing having an

outer portion with a ruthenium-based fluorescing chemistry thereon,

the outer portion spatially arranged to;

receive light from the diode, thereby exciting the fluorescing chemistry,

reflect a substantial portion of the light received from the diode away from the photodiode; and

allow light from the fluorescing chemistry to impinge upon the photodiode.

8. The sensor according to claim 7, further comprising an optical sink arranged within the housing to minimize the light from the diode which is incident upon the photodiode.

9. The sensor according to claim 7, wherein the ruthenium-based chemistry exists in a sol-gel chemistry layer.