×

Surface expression of enzyme in gene directed prodrug therapy

  • US 6,025,340 A
  • Filed: 01/31/1997
  • Issued: 02/15/2000
  • Est. Priority Date: 07/27/1994
  • Status: Expired due to Term
First Claim
Patent Images

1. A product comprising two components for use in association with one another comprising:

  • (a) a vector comprising a eukaryotic promoter operably linked to a gene, said gene comprising a nucleic acid sequence encoding a bacterial carboxypeptidase G which has been altered at one or more glycosylation sites to prevent glycosylation at said site or sites together with a signal sequence, wherein upon expression of said gene in a eukaryotic tumor cell said signal sequence directs and tethers said bacterial carboxypeptidase to the plasma membrane of said tumor cell; and

    (b) a prodrug which can be converted into an active drug by said bacterial carboxypeptidase G.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×