Fluorescence detecting system
First Claim
1. A fluorescence detecting system for detecting auto fluorescence emitted from an intrinsic pigment in a part of an organism to be observed comprising:
- an excitation light projecting means which intermittently projects onto the part to be observed excitation light in a wavelength range which can excite the intrinsic pigment of the organism to emit auto fluorescence,a fluorescence detecting means which extracts an auto fluorescence component in a desired wavelength range from auto fluorescence emitted from the intrinsic pigment,a base line component removing means which obtains a net auto fluorescence component in the desired wavelength range by subtracting a base line component in the desired wavelength range the fluorescence detecting means detects when the part of the organism is not exposed to the excitation light from a gross auto fluorescence component in the desired wavelength range the fluorescence detecting means detects when the part of the organism is exposed to the excitation light, anda divider means which carries out a division between a first part of the net auto fluorescence component in the desired wavelength range and a second part of the net auto fluorescence component in the desired wavelength range.
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Accused Products
Abstract
A fluorescence detecting system detects auto fluorescence emitted from an intrinsic pigment in a part of an organism. An excitation light projector intermittently projects onto the part excitation light in the wavelength range which can excite the intrinsic pigment of the organism to emit auto fluorescence. A fluorescence detector extracts an auto fluorescence component in a desired wavelength range from auto fluorescence emitted from the pigment. A net auto fluorescence component in the desired wavelength range is obtained by subtracting a base line component in the desired wavelength range the fluorescence detector detects when the part of the organism is not exposed to the excitation light from a gross auto fluorescence component in the desired wavelength range the fluorescence detector detects when the part of the organism is exposed to the excitation light. A divider carries out a division between a first part of the net auto fluorescence component in the desired wavelength range and a second part of the same.
89 Citations
20 Claims
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1. A fluorescence detecting system for detecting auto fluorescence emitted from an intrinsic pigment in a part of an organism to be observed comprising:
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an excitation light projecting means which intermittently projects onto the part to be observed excitation light in a wavelength range which can excite the intrinsic pigment of the organism to emit auto fluorescence, a fluorescence detecting means which extracts an auto fluorescence component in a desired wavelength range from auto fluorescence emitted from the intrinsic pigment, a base line component removing means which obtains a net auto fluorescence component in the desired wavelength range by subtracting a base line component in the desired wavelength range the fluorescence detecting means detects when the part of the organism is not exposed to the excitation light from a gross auto fluorescence component in the desired wavelength range the fluorescence detecting means detects when the part of the organism is exposed to the excitation light, and a divider means which carries out a division between a first part of the net auto fluorescence component in the desired wavelength range and a second part of the net auto fluorescence component in the desired wavelength range. - View Dependent Claims (2, 3, 4, 5)
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6. A fluorescence detecting system for detecting fluorescence emitted from a part of an organism to be observed which has been given a fluorescent photosensitive material comprising:
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an excitation light projecting means which intermittently projects onto the part to be observed excitation light in a wavelength range which can excite an intrinsic pigment of the organism and said fluorescent photosensitive material to emit fluorescence, a fluorescence detecting means which extracts a fluorescence component in a desired wavelength range from fluorescence emitted from the intrinsic pigment and the fluorescent photosensitive material, a base line component removing means which obtains a net fluorescence component in the desired wavelength range by subtracting a base line component in the desired wavelength range the fluorescence detecting means detects when the part of the organism is not exposed to the excitation light from a gross fluorescence component in the desired wavelength range which the fluorescence detecting means detects when the part of the organism is exposed to the excitation light, and a divider means which carries out a division between a first part of the net fluorescence component in the desired wavelength range and a second part of the net fluorescence component in the desired wavelength range. - View Dependent Claims (7, 8, 9, 10)
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11. A fluorescence detecting system for detecting auto fluorescence emitted from an intrinsic pigment in a part of an organism to be observed comprising:
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an excitation light projector which intermittently projects excitation light onto the part of the organism to be observed in a wavelength range which can excite the intrinsic pigment of the organism to emit auto fluorescence, a fluorescence detector which extracts an auto fluorescence component in a desired wavelength range from the auto fluorescence emitted from the intrinsic pigment and outputs a first signal, a base line component removing circuit comprising a subtractor which inputs a second signal when the organism is not exposed to said excitation light, said second signal defined as a base line component, and which inputs said first signal when the organism is exposed to said excitation light, and which takes the difference of the first and second signals, and thereby obtains a net auto fluorescence component in the desired wavelength range, and a divider which carries out a division between a first part of the net auto fluorescence component in the desired wavelength range and a second part of the net auto fluorescence component in the desired wavelength range. - View Dependent Claims (12, 13, 14, 15)
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16. A fluorescence detecting system for detecting fluorescence emitted from a part of an organism to be observed which has been given a fluorescent photosensitive material comprising
an excitation light projector which intermittently projects excitation light onto the part of the organism to be observed in a wavelength range which can excite an intrinsic pigment of the organism and said fluorescent photosensitive material to emit fluorescence, a fluorescence detector which extracts a fluorescence component in a desired wavelength range from fluorescence emitted from the intrinsic pigment as well as the fluorescent photosensitive material, and outputs a first signal, a base line component removing circuit comprising a subtractor which inputs a second signal when the organism is not exposed to said excitation light, said second signal defined as a base line component, and which inputs said first signal when the organism is exposed to said excitation light, and which takes the difference of the first and second signals, and thereby obtains a net auto fluorescence component in the desired wavelength range, and a divider which carries out a division between a first part of the net auto fluorescence component in the desired wavelength range and a second part of the net auto fluorescence component in the desired wavelength range.
Specification