Spillproof microplate assembly

• US 6,027,694 A
• Filed: 10/17/1997
• Issued: 02/22/2000
• Est. Priority Date: 10/17/1996
• Status: Expired due to Fees

• Current Assignee: Texperts, Inc.
• Original Assignee: Texperts, Inc.
• Inventors: Boulton, David A., Solewski, Carolyn I.
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/953,441
• Time in Patent Office: 858 Days
• Field of Search: 422/102, 422/104, 422/99-101, 435/286.6, 435/288.2-288.4
• US Class Current: 422/553
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00313 the reactor vessels being formed by arrays of wells in blocks
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00335 Septa
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00587 High throughput processes
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

A microplate assembly comprising a multi-well microplate, a plurality of vent caps and a porous vent film. The microplate includes a frame that houses a plurality of open wells in a rectangular array. Vent caps mount on the microplate to seal and vent the wells. When the vent caps are coupled to the wells, an interior volume is formed in each well. The wells function as a vessel for liquid samples that occupy predetermined spaces within the interior volumes. The liquid samples remain within its predetermined space for all orientations of the microplate assembly. The vent cap comprises an array of well inserts. Each well insert comprises a sealing plug and a vent tube. The well inserts are interconnected by a perforated web. The sealing plugs form a seal at the mouth of the open wells. The vent tubes, which are fixed to the sealing plugs, terminate in a vent that communicates with the interior volumes outside the predetermined spaces occupied by the liquid samples. The vents permit the pressure within the interior volume to be equalized with the ambient pressure via a passage that runs through the vent tube and the sealing plugs. Material may be added to or removed from each well without removing the vent cap. The porous vent film, which has an adhesive coating, adheres to the vent caps while covering the passages, thereby inhibiting evaporation of the liquid samples.

Claims

1. A liquid holding apparatus comprising:

a fluid vessel having an opening and an interior volume with a liquid-holding space located therein; and

a vent cap having means for equalizing gas pressure between said interior volume and the exterior of said fluid vessel and for sealing said opening against the independent passage of liquid from said interior volume to said exterior of said vessel for all possible positions of said vessel, said vent cap including;

a press-fit plug mounted in said opening and having a flexible body with a resilient outer surface forming a friction-tight impermeable seal with an inside surface of said opening,

a vent tube having first and second ends, said first end of said vent tube fixed to said press-fit plug and communicating with the exterior of said vessel, said second end of said vent tube extending into said interior volume and comprising a vent hole communicating with said interior volume outside said liquid-holding space for all said possible positions of said vessel, and

a passage running from said vent hole through said vent tube and said body of said press-fit plug.

2. The apparatus of claim 1 wherein said vent hole is substantially located at the centroid of said interior volume.

3. The apparatus of claim 2 further including a porous vent film mounted on said press-fit plug and covering said passage, and wherein said press-fit plug and said vent tube are molded as a unitary structure.

4. A microplate assembly comprising:

a microplate having a rigid body with a plurality of open wells mounted therein, each of said open wells comprising a fluid vessel with an opening and an interior volume with a liquid-holding space located therein; and

a vent cap having means for equalizing gas pressure between said interior volume and the exterior of said vessel and for sealing the plurality of open wells simultaneously to prevent the independent passage of liquid from said interior volume to said exterior of said vessel for all possible orientations of said vessel, the vent cap including;

a plurality of press-fit plugs each having a flexible body with a resilient outer surface mounted in a different one of said openings, and each said resilient outer surface forming a friction-tight impermeable seal with an inside surface of a corresponding one of said openings,

vent tubes each having first and second ends, said first end of each said vent tube fixed to a different one of said press-fit plugs and communicating with the exterior of said vessel, said second end of each said vent tube comprising a vent hole communicating with the interior volume outside said liquid-holding space for all possible orientations of said assembly,

a passage running from said vent hole through said vent tube and said body of each said press-fit plug, and

a flexible sheet connected to said plurality of press-fit plugs.

5. The microplate assembly of claim 4 wherein said vent hole is substantially located at the centroid of said interior volume.

6. The microplate assembly of claim 5 wherein said openings define a first plane and said flexible sheet defines a second plane parallel to and superimposed on said first plane, and further including perforations located in said flexible sheet to form a planar web connected to said resilient outer surface of each said press-fit plug such that said passages are substantially parallel to each other and perpendicular to said first plane.

7. The microplate assembly of claim 6 further including a porous vent film mounted on said press-fit plugs and covering said passages, and wherein said vent cap is molded as a unitary structure.

8. A vent cap for sealing an opening in a fluid vessel having an interior volume with a liquid-holding space located therein, said vent cap equalizing gas pressure between said interior volume and the exterior of said vessel and preventing the independent passage of liquid from said interior volume to said exterior of said vessel for all possible positions of said vessel, said vent cap comprising:

a press-fit plug having a flexible body with a resilient outer surface capable of forming a friction-tight impermeable seal with an inside surface of said opening;

a vent tube having first and second ends, said first end of said vent tube fixed to said press-fit plug to communicate with said exterior of said vessel, said second end of said vent tube comprising a vent hole to communicate with said interior volume outside said liquid-holding space for said all possible positions of said vessel; and

a passage running from said vent hole through said vent tube and said body of said press-fit plug.

9. The vent cap of claim 8 further including a porous vent film mounted on said press-fit plug and covering said passage.

10. The vent cap of claim 9 wherein said press-fit plug and said vent tube are molded as a unitary structure.

11. A vent cap for sealing openings in multiple open fluid vessels arranged in a predetermined pattern, each said fluid vessel having an interior volume with a liquid-holding space located therein, said vent cap equalizing gas pressure between said interior volumes and the exterior of said vessels and preventing the independent passage of liquid from said interior volumes to said exterior of said vessels for all possible positions of said vessels, said vent cap comprising:

a plurality of press-fit plugs each having a flexible body with a resilient outer surface capable of forming a friction-tight impermeable seal with an inside surface of said opening;

a plurality of vent tubes each having first and second ends, said first end of each said vent tube fixed to a different one of said press-fit plugs for communicating with said exterior of said vessels, said second end of each said vent tub comprising a vent hole communicating with said interior volume outside said liquid-holding space for said all possible positions of said vessels;

a passage running from each said vent hole through each said vent tube and each said press-fit plug; and

a flexible sheet connected to said plurality of press-fit plugs and holding said press-fit plugs in a pattern corresponding to said predetermined pattern.

12. The vent cap of claim 11 further including at least one pull tab mounted on the periphery of said flexible sheet, and wherein said flexible sheet includes perforations to form a planar web connected to said press-fit plugs, said web mounting said press-fit plugs in a common plane such that the longitudinal axes of said vent tubes are parallel to each other and perpendicular to said common plane.

13. The vent cap of claim 12 wherein said web, said press-fit plugs and said vent tubes are molded into a unitary structure.

14. A method of sealing a vessel, to prevent the independent passage of liquid from said vessel for all possible positions of said vessel, and venting said vessel, to equalize the gas pressure between the interior and exterior of said vessel, and wherein said vessel has an opening and an interior volume with a liquid-holding space located therein, said method comprising:

inserting a press-fit plug having a flexible body with a resilient outer surface in said opening to form a friction-tight impermeable seal between said outer surface and an inside surface of said opening;

forming a vent hole in said interior of said vessel outside said liquid holding space for all possible positions of said vessel; and

extending a tubular passage from said vent hole through said flexible body of said press-fit plug to the exterior of said vessel such that said vent hole communicates with said interior volume outside said liquid-holding space and said exterior of said vessel.

15. The method of claim 14 further including placing a liquid sample in said liquid-holding space in said interior volume such that said liquid sample occupies said liquid-holding space for all orientations of said vessel, and wherein said forming step includes placing said vent hole in communication with said interior volume outside said liquid-holding space.

16. The method of claim 15 wherein said forming step includes placing said vent hole at substantially the centroid of said interior volume.

17. A method of sealing and venting multiple open wells contained in a multi-well microplate, each said well comprising a fluid vessel with an opening, an interior volume and a liquid-holding space located in said interior volume, said wells being sealed to prevent the independent passage of liquid from said liquid-holding space to the exterior of said vessel, said method comprising the steps of:

inserting a press-fit plug having a flexible body with a resilient outer surface in each of said openings to form a friction-tight impermeable seal between said outer surface and an inside surface of said opening;

forming a vent hole in the interior volume of each of said wells outside said liquid-holding spaces for all possible positions of said wells;

extending a passage from each said vent hole through the flexible body of each said press-fit plug to the exterior of said wells; and

joining said press-fit plugs to each other with a flexible sheet in a planar array to match a pattern of said open wells.

18. The method of claim 17 further including placing a liquid sample in each said liquid-holding space for all orientations of said microplate, and wherein said forming step includes placing each said vent hole in communication with said interior volume outside said liquid-holding space.

19. The method of claim 18 wherein said forming step includes placing each said vent hole at substantially the centroid of said interior volume.