Guided development and support of hydrogel-cell compositions

• US 6,027,744 A
• Filed: 04/24/1998
• Issued: 02/22/2000
• Est. Priority Date: 04/24/1998
• Status: Expired due to Term

• Current Assignee: Children's Medical Center Corporation, Vbi Technologies, LLC
• Original Assignee: Children's Medical Center Corporation, University Of Massachusetts
• Inventors: Vacanti, Joseph P., Vacanti, Charles A.
• Primary Examiner(s): Azpuru, Carlos A.
• Application Number:
  US09/066,038
• Time in Patent Office: 669 Days
• Field of Search: 424/426, 623/12, 623/16
• US Class Current: 424/426
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61F 2002/30062 (bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive
• CPC Class Codes: A61F 2002/30199 Three-dimensional shapes
• CPC Class Codes: A61F 2210/0004 bioabsorbable
• CPC Class Codes: A61F 2230/0063 Three-dimensional shapes
• CPC Class Codes: A61F 2310/00293 containing a phosphorus-containing compound, e.g. apatite
• CPC Class Codes: A61L 27/12 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids

Abstract

The invention features a method for generating new tissue, the method including: obtaining a liquid hydrogel-cell composition including a hydrogel and tissue precursor cells; delivering the liquid hydrogel-cell composition into a permeable, biocompatible support structure; and allowing the liquid hydrogel-cell composition to solidify within the support structure and the tissue precursor cells to grow and generate new tissue. The invention also features a tissue forming structure including: a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue; and a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells.

Claims

1. A method for generating new tissue, the method comprising:

obtaining a liquid hydrogel-cell composition comprising a hydrogel and tissue precursor cells;

delivering the liquid hydrogel-cell composition into a permeable, biocompatible support structure; and

allowing the liquid hydrogel-cell composition to solidify within the support structure and the tissue precursor cells to grow and generate new tissue that can be vascularized.

2. The method of claim 1, wherein the delivered liquid hydrogel-cell composition is injected into the support structure.

3. The method of claim 1, further comprising implanting the support structure into an animal.

4. The method of claim 3, wherein the hydrogel-cell composition is delivered after the support structure is implanted into an animal.

5. The method of claim 1, wherein the support structure comprises a ceramic material.

6. The method of claim 1, wherein the support structure is shaped in the form of desired tissue.

7. The method of claim 6, wherein the support structure is shaped in the form of articular cartilage adjacent a joint, a bone, a portion of a bone, or a bone defect.

8. The method of claim 1, wherein the support structure is biodegradable.

9. The method of claim 1, wherein the support structure comprises a sponge or foam.

10. The method of claim 1, wherein the support structure is compressible.

11. The method of claim 1, wherein the support structure comprises a mesh of fibers.

12. The method of claim 1, wherein the support structure is rigid.

13. The method of claim 1, wherein the support structure is formed from polyanhydride, polyorthoester, polyglycolic acid, polylactic acid, or polyglactin.

14. The method of claim 1, wherein the support structure comprises porous hydroxyapatite.

15. The method of claim 1, wherein the hydrogel is selected from the group consisting of polysaccharides, proteins, polyphosphazenes, poly(oxyethylene)-poly(oxypropylene) block polymers, poly(oxyethylene)-poly(oxypropylene) block polymers of ethylene diamine, poly(acrylic acids), poly(methacrylic acids), copolymers of acrylic acid and methacrylic acid, poly(vinyl acetate), and sulfonated polymers.

16. The method of claim 1, wherein the tissue precursor cells are selected from the group consisting of epidermal cells, chondrocytes, cartilage forming cells, macrophages, dermal cells, muscle cells, hair follicles, fibroblasts, organ cells, osteoblasts, bone forming cells, endothelial cells, mucosal cells, pleural cells, ear canal cells, tympanic membrane cells, peritoneal cells, Schwann cells, corneal epithelial cells, gingiva cells, neural cells, neural stem cells, and tracheal epithelial cells.

17. A tissue forming structure comprising:

a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue; and

a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells, and wherein the support structure is sufficiently permeable to permit the tissue precursor cells to grow, become vascularized, and generate new tissue.

18. The tissue forming structure of claim 17, wherein the hydrogel-cell composition is a solidified suspension of hydrogel supporting dispersed tissue precursor cells.

19. The tissue forming structure of claim 17, wherein the support structure comprises a ceramic material.

20. The tissue forming structure of claim 17, wherein the support structure is biodegradable.

21. The tissue forming structure of claim 17, wherein the support structure comprises a sponge or foam.

22. The tissue forming structure of claim 17, wherein the support structure is compressible.

23. The tissue forming structure of claim 17, wherein the support structure comprises a mesh of polymeric fibers.

24. The tissue forming structure of claim 17, wherein the support structure is formed from polyanhydride, polyorthoester, polyglycolic acid, polylactic acid, or polyglactin.

25. The tissue forming structure of claim 17, wherein the support structure comprises porous hydroxyapatite.

26. The tissue forming structure of claim 17, wherein the support structure comprises metal.

27. The tissue forming structure of claim 17, wherein the support structure is rigid.

28. The tissue forming structure of claim 17, wherein the hydrogel is selected from the group consisting of polysaccharides, proteins, polyphosphazenes, poly(oxyethylene)-poly(oxypropylene) block polymers, poly(oxyethylene)-poly(oxypropylene) block polymers of ethylene diamine, poly(acrylic acids), poly(methacrylic acids), copolymers of acrylic acid and methacrylic acid, poly(vinyl acetate), and sulfonated polymers.

29. The tissue forming structure of claim 17, wherein the tissue precursor cells are selected from the group consisting of epidermal cells, chondrocytes, cartilage forming cells, macrophages, dermal cells, muscle cells, hair follicles, fibroblasts, organ cells, osteoblasts, bone forming cells, endothelial cells, mucosal cells, pleural cells, ear canal cells, tympanic membrane cells, peritoneal cells, Schwann cells, corneal epithelial cells, gingiva cells, neural cells, neural stem cells, and tracheal epithelial cells.

30. A tissue forming structure of claim 17, wherein the cells are bone forming cells and the support structure comprises porous hydroxyapatite.

31. A method for generating new tissue, the method comprising:

obtaining a liquid hydrogel-cell composition comprising a hydrogel and tissue precursor cells;

delivering the liquid hydrogel-cell composition into a permeable, biocompatible support structure shaped in the form of desired tissue; and

allowing the liquid hydrogel-cell composition to solidify within the support structure and the tissue precursor cells to grow and generate new tissue.

32. The method of claim 31, wherein the delivered liquid hydrogel-cell composition is injected into the support structure.

33. The method of claim 31, further comprising implanting the support structure into an animal.

34. The method of claim 33, wherein the hydrogel-cell composition is delivered after the support structure is implanted into an animal.

35. The method of claim 31, wherein the support structure is shaped in the form of articular cartilage adjacent a joint, a bone, a portion of a bone, or a bone defect.

36. A tissue forming structure comprising:

a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue, the support structure comprising a ceramic material; and

a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells.

37. A tissue forming structure comprising:

a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue, the support structure comprising a sponge or foam; and

a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells.

38. A tissue forming structure comprising:

a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue, the support structure comprising a mesh of polymeric fibers; and

a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells.

39. A tissue forming structure comprising:

a permeable, biocompatible support structure having a predetermined shape that corresponds to the shape of desired tissue, the support structure comprising porous hydroxyapatite; and

a hydrogel-cell composition at least partially filling the support structure, wherein the hydrogel-cell composition comprises a hydrogel and tissue precursor cells.