Platelet count assay using thrombospondin or .beta.-thromboglobulin

• US 6,027,904 A
• Filed: 10/09/1997
• Issued: 02/22/2000
• Est. Priority Date: 03/29/1996
• Status: Expired due to Fees

• Current Assignee: British Columbia Cancer Agency Branch (Province of British Columbia)
• Original Assignee: University of British Columbia
• Inventors: Devine, Dana Virginia, Brooks, Donald Elliott
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): GRUN, JAMES LESLIE
• Application Number:
  US08/947,981
• Time in Patent Office: 866 Days
• Field of Search: 435/7.2, 435/7.21, 435/7.9, 435/7.92, 435/7.93, 435/7.94, 435/13, 435/970, 435/287.2, 435/287.3, 435/287.7, 435/287.8, 435/287.9, 435/288.6, 435/288. 7, 436/514, 436/524, 436/528, 436/531, 436/533, 436/534, 436/548, 436/69, 436/87, 436/164, 436/169, 436/170, 436/172, 436/174, 436/807, 436/536, 422/56, 422/57
• US Class Current: 435/7.21
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 33/86 involving blood coagulating time or factors, or their receptors
• CPC Class Codes: Y10S 435/97 Test strip or test slide

Abstract

Methods of calculating the platelet count of an individual, by measuring the amount of a released platelet granule protein of interest in a sample of whole blood or of platelet-rich plasma from the individual, are described. The platelet granule protein of interest is either thrombospondin or β-thromboglobulin. The amount of released platelet granule protein of interest in the whole blood sample or platelet-rich plasma sample is measured using an enzyme-linked immunosorbent assay; radioimmunoassay; sandwich assay; a quantitative immunochromatographic assay; or non-solid phase nephelometry. The platelet count is directly related to the amount of released platelet granule protein of interest in the sample, and can be determined from the amount of platelet granule protein of interest that is released from a known number of platelets.

Claims

1. A method of determining platelet count of an individual, comprising the steps of:

a) releasing platelet granule proteins from platelets in a blood sample from the individual, thereby generating a test sample comprising an amount of a released platelet granule protein of interest;

b) measuring the amount of the released platelet granule protein of interest in the test sample, the released platelet granule protein of interest being selected from the group consisting of thrombospondin and β

-thromboglobulin; and

c) determining platelet count of the individual from the measured amount of the released platelet granule protein of interest in the test sample.

2. The method of claim 1, wherein the amount of the released platelet granule protein of interest is measured by an enzyme-linked immunosorbent assay.

3. The method of claim 1, wherein the amount of the released platelet granule protein of interest is measured by non-solid phase nephelometry.

4. The method of claim 1, wherein the platelet count is calculated from a standard curve.

5. A method of determining platelet count of an individual, comprising the steps of:

a) isolating a platelet-rich plasma sample from a blood sample from the individual;

b) releasing platelet granule proteins from platelets in the platelet-rich plasma sample, thereby generating a test sample comprising an amount of a released platelet granule protein of interest;

c) measuring the amount of the released platelet granule protein of interest in the test sample, the released platelet granule protein of interest being selected from the group consisting of thrombospondin and β

-thromboglobulin; and

d) determining the platelet count of the individual from the measured amount of the released platelet granule protein of interest in the test sample.

6. The method of claim 5, wherein the amount of the released platelet granule protein of interest is measured by an enzyme-linked immunosorbent assay.

7. The method of claim 5, wherein the amount of the released platelet granule protein is measured by non-solid phase nephelometry.

8. The method of claim 5, wherein the platelet count is calculated from a standard curve.