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Methods of isolating biological target materials using silica magnetic particles

  • US 6,027,945 A
  • Filed: 01/21/1997
  • Issued: 02/22/2000
  • Est. Priority Date: 01/21/1997
  • Status: Expired due to Term
First Claim
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1. A method for isolating a biological target material from other material in a medium by:

  • a. providing a medium including the biological target material;

    b. providing silica magnetic particles capable of directly and reversibly binding the biological target material in the absence of an intermediary substance;

    c. forming a complex of the silica magnetic particles and the biological target material by combining the silica magnetic particles and the medium, the biological target material being directly adhered to the silica magnetic particles;

    d. removing the complex from the medium by application of an external magnetic field; and

    e. separating the biological target material from the complex by eluting the biological target material, whereby the isolated biological target material is obtained.

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