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Probes labeled with energy transfer coupled dyes

  • US 6,028,190 A
  • Filed: 05/08/1996
  • Issued: 02/22/2000
  • Est. Priority Date: 02/01/1994
  • Status: Expired due to Term
First Claim
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1. A fluorescent label comprising:

  • at least two fluorophores bonded to a polymeric backbone at specific locations thereon to form a chemically pure label wherein at least one fluorophore is bonded to a terminal monomeric unit of said polymeric backbone and the other fluorophore(s) is bonded to a non-terminal monomeric unit of said polymeric backbone and said terminally bonded fluorophore is in an energy transfer relationship with said other fluorophore(s), said fluorophores comprising a donor fluorophore and an acceptor fluorophore of which said donor fluorophore absorbs light in the wavelength range of 350 to 800 nm and said acceptor emits light in the wavelength range of 450 to 1000 nm.

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