Plant C18:1 preferring thioesterases
First Claim
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1. A recombinant DNA construct comprising a plant C18:
- 1 preferring acyl-ACP thioesterase encoding sequence, wherein said C18;
1 preferring thioesterase is from safflower or Brassica.
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Abstract
This invention relates to plant thioesterases, means to identify such proteins, amino acid and nucleic acid sequences associated with such protein, methods to obtain, make and/or use such plant thioesterases. Also, by this invention, the existence of a heretofore unproven factor critical to the biosynthesis of medium-chain fatty acids in plants is demonstrated.
4 Citations
17 Claims
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1. A recombinant DNA construct comprising a plant C18:
- 1 preferring acyl-ACP thioesterase encoding sequence, wherein said C18;
1 preferring thioesterase is from safflower or Brassica. - View Dependent Claims (16, 17)
- 1 preferring acyl-ACP thioesterase encoding sequence, wherein said C18;
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2. A recombinant DNA construct comprising as operably linked components in the 5'"'"' to 3'"'"' direction of transcription, a transcriptional initiation region functional in a host plant or bacterial cell and a plant C18:
- 1 preferring acyl-ACP thioesterase nucleic acid sequence, wherein said transcriptional initiation region is not naturally linked to said thioesterase sequence, and wherein said C18;
1 preferring thioesterase is from safflower or Brassica. - View Dependent Claims (3, 4)
- 1 preferring acyl-ACP thioesterase nucleic acid sequence, wherein said transcriptional initiation region is not naturally linked to said thioesterase sequence, and wherein said C18;
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5. A DNA construct comprising an expression cassette capable of producing a plant thioesterase in a plant cell comprising as operably linked components in the 5'"'"' to 3'"'"' direction of transcription, a transcriptional initiation regulatory region functional in said plant cell, a translational initiation regulatory region functional in said plant cell, a sense DNA sequence encoding a biologically active plant C18:
- 1 preferring acyl-ACP thioesterase, and a transcriptional termination regulatory region functional in said plant cell, wherein at least one of said regulatory regions is not naturally linked to said sense DNA sequence, and wherein said C18;
1 preferring thioesterase is from safflower or Brassica. - View Dependent Claims (6, 7, 8, 9, 10, 11)
- 1 preferring acyl-ACP thioesterase, and a transcriptional termination regulatory region functional in said plant cell, wherein at least one of said regulatory regions is not naturally linked to said sense DNA sequence, and wherein said C18;
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12. A method of modifying the fatty acid composition in a plant cell comprising:
growing a plant cell having integrated in its genome a DNA construct, said construct comprising in the 5'"'"' to 3'"'"' direction of transcription, a transcriptional regulatory region functional in said plant cell and a plant C18;
1 preferring acyl-ACP thioesterase encoding sequence, under conditions which will permit the transcription of said plant C18;
1 preferring acyl-ACP thioesterase.- View Dependent Claims (13, 14, 15)
Specification