Method for the detection of malignant and premalignant stages of cervical cancer
First Claim
1. A method for the identification of a malignant or premalignant condition in an exfoliated cervical cell sample, said method comprising:
- (a) drying said exfoliated cervical cell sample on an infrared transparent matrix to produce a dried cell sample;
(b) directing a beam of mid-infrared light at said dried cell sample, said beam of mid-infrared light having a frequency of from about 3000 to about 950 cm-1 to produce absorption data for said dried cell sample; and
(c) comparing said absorption data for said dried cell sample with a calibration/reference set of infrared absorption data to determine whether variation in infrared absorption occurs in said dried cell sample, at at least one range of frequencies, due to the said variation being characteristic of said malignant or premalignant condition, said comparing utilizing a partial least squares or principal component analysis statistical method and said absorption data being underivatized and unsmoothed, whereby said identification of said malignant or premalignant condition is made.
5 Assignments
0 Petitions
Accused Products
Abstract
This invention discloses a method to identify premalignant and malignant stages of cervical cancer from an infrared (IR) spectrum of exfoliated cervical cells which are dried on an infrared transparent matrix and scanned at the frequency range from 3000-950 cm-1. The identification of samples is based on establishing a calibration using a representative set of spectra of normal, dysplastic and malignant specimens. During the calibration process, multivariate techniques such as Principal Component Analysis (PCA) and/or Partial Least Squares (PLS) are used. PCA and PLS reduce the data based on maximum variations between the spectra, and generate clusters in a multidimensional space representing the different populations. The utilization of Mahalinobis distances, or linear regression (e.g., Principle Component Regression on the reduced data from PCA) form the basis for the discrimination. This method is simple to use and achieves statistically reliable distinction between the following groups of cervical smears: normal (individuals with no prior history of dysplasia), dysplasia and malignant samples. Lastly, this invention discloses a method to obtain the IR spectrum of individual cervical cells fixed on an infrared transparent matrix.
-
Citations
12 Claims
-
1. A method for the identification of a malignant or premalignant condition in an exfoliated cervical cell sample, said method comprising:
-
(a) drying said exfoliated cervical cell sample on an infrared transparent matrix to produce a dried cell sample; (b) directing a beam of mid-infrared light at said dried cell sample, said beam of mid-infrared light having a frequency of from about 3000 to about 950 cm-1 to produce absorption data for said dried cell sample; and (c) comparing said absorption data for said dried cell sample with a calibration/reference set of infrared absorption data to determine whether variation in infrared absorption occurs in said dried cell sample, at at least one range of frequencies, due to the said variation being characteristic of said malignant or premalignant condition, said comparing utilizing a partial least squares or principal component analysis statistical method and said absorption data being underivatized and unsmoothed, whereby said identification of said malignant or premalignant condition is made. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
Specification