Condensate colorimetric nitrogen oxide analyzer
First Claim
1. A device for determination of a content of higher oxides of nitrogen in exhaled breath condensate comprising:
- a conduit for receiving a flow of exhalate, said conduit having an exhalate condensing portion with an inlet, an outlet and a gas release port coupled to the outlet;
a coolant jacket coaxially surrounding said exhalate condensing portion; and
a sample assay assembly in communication with said conduit exhalate condensing portion outlet, wherein the sample assay assembly comprises;
(i) a light transmissive analysis chamber attached to the outlet to receive the exhaled breath condensate, wherein the light transmissive analysis chamber comprises a reagent entry port,(ii) a reagent chamber in communication with the reagent entry port, and(iii) a pliable element connecting the outlet and the analysis chamber and forming a portion of the reagent chamber, wherein flexion of the pliable element closes communication between the outlet and the analysis chamber and contracts the reagent chamber to deliver to the analysis chamber controlled amounts of condensate and reagent.
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Accused Products
Abstract
Disclosed is a device for determination of the content of higher oxides of nitrogen in exhaled breath condensate which comprises a conduit having an exhalate condensing portion with an inlet and an outlet (the inlet canbe configured to fit with a mechanical respirator or, for direct use by the patient, an inlet assembly providing one-way ingress of ambient atmosphere to the device can be associated with the inlet of the conduit exhalate condensing portion); a coolant jacket coaxially surrounding said exhalate condensing portion; a gas release port; and, in enclosed fluid communication with said conduit exhalate condensing portion outlet, a sample assay assembly comprising (i) a translucent analysis chamber attached to the outlet to receive condensate fluid and having a reagent entry port, (ii) a reagent chamber in enclosed fluid communication with the reagent entry port and (iii) a pliable element connecting the outlet and the analysis chamber and forming a portion of the reagent chamber such that flexion of the pliable element closes communication between the outlet and the analysis chamber and contracts the reagent chamber so as to deliver to the analysis chamber controlled amounts of condensate and reagent. The device is disposable and inexpensive, and is used to collect human exhalate for calorimetric assay of liquid and gas phase nitrogen oxides to assist in evaluation of airway inflammation.
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Citations
26 Claims
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1. A device for determination of a content of higher oxides of nitrogen in exhaled breath condensate comprising:
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a conduit for receiving a flow of exhalate, said conduit having an exhalate condensing portion with an inlet, an outlet and a gas release port coupled to the outlet; a coolant jacket coaxially surrounding said exhalate condensing portion; and a sample assay assembly in communication with said conduit exhalate condensing portion outlet, wherein the sample assay assembly comprises; (i) a light transmissive analysis chamber attached to the outlet to receive the exhaled breath condensate, wherein the light transmissive analysis chamber comprises a reagent entry port, (ii) a reagent chamber in communication with the reagent entry port, and (iii) a pliable element connecting the outlet and the analysis chamber and forming a portion of the reagent chamber, wherein flexion of the pliable element closes communication between the outlet and the analysis chamber and contracts the reagent chamber to deliver to the analysis chamber controlled amounts of condensate and reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A device for determining a content of higher oxides of nitrogen in exhaled breath condensate comprising:
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a conduit for receiving a flow of exhalate, said conduit having an exhalate condensing portion with an inlet, an outlet and a gas release port coupled to the outlet; a coolant jacket coaxially surrounding said exhalate condensing portion; and a sample assay assembly in communication with said conduit exhalate condensing portion outlet, wherein the sample assay assembly comprises; (i) a light transmissive analysis chamber attached to the outlet to receive exhaled breath condensate, wherein the light transmissive analysis chamber comprises a reagent entry port, (ii) a reagent chamber in communication with the reagent entry port, wherein the reagent entry port comprises a reagent-permeable membrane, and (iii) a pliable element connecting the outlet and the analysis chamber and forming a portion of the reagent chamber, wherein flexion of the pliable element closes communication between the outlet and the analysis chamber and contracts the reagent chamber to deliver to the analysis chamber controlled amounts of condensate and reagent. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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19. A device for determining a content of higher oxides of nitrogen in exhaled breath condensate comprising:
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a conduit for receiving a flow of exhalate, said conduit having an exhalate condensing portion with an inlet, an outlet and a gas release port coupled to the outlet; a coolant jacket coaxially surrounding said exhalate condensing portion; and a sample assay assembly in communication with said conduit exhalate condensing portion outlet, wherein the sample assay assembly comprises; (i) a light transmissive analysis chamber attached to the outlet to receive exhaled breath condensate, wherein the light transmissive analysis chamber comprises a reagent entry port, (ii) a reagent chamber in communication with the reagent entry port, wherein the reagent entry port comprises a rupturable membrane, and (iii) a pliable element connecting the outlet and the analysis chamber and forming a portion of the reagent chamber, wherein flexion of the pliable element closes communication between the outlet and the analysis chamber and contracts the reagent chamber to deliver to the analysis chamber controlled amounts of condensate and reagent. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
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Specification