Microplates which prevent optical cross-talk between wells
First Claim
1. A method of making a microplate having a plurality of wells, each of the plurality of wells having light impermeable sidewalls and a light permeable bottom wall, the method comprising the steps of:
- (A) inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, the plate being positioned adjacent the sections shaped to form the sidewalls of the plurality of wells in a location corresponding to the bottom walls of the plurality of wells;
(B) injecting molten light impermeable material into the mold cavity; and
(C) cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells.
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Accused Products
Abstract
A microplate and a method for manufacturing microplates. The microplate is designed to reduce optical cross-talk during the assaying of samples. The microplate includes a plurality of wells having light permeable sidewalls, a layer of light permeable material forming a bottom of the wells, and ribs of light impermeable material extending through the layer of light permeable material. The method includes steps of inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten light impermeable material into the mold cavity, and cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells.
50 Citations
17 Claims
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1. A method of making a microplate having a plurality of wells, each of the plurality of wells having light impermeable sidewalls and a light permeable bottom wall, the method comprising the steps of:
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(A) inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, the plate being positioned adjacent the sections shaped to form the sidewalls of the plurality of wells in a location corresponding to the bottom walls of the plurality of wells; (B) injecting molten light impermeable material into the mold cavity; and (C) cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells. - View Dependent Claims (2)
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3. A method of making a microplate having a plurality of wells, each of the plurality of wells having light impermeable sidewalls and a light permeable bottom wall, the method comprising the steps of:
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(A) inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, the plate being positioned adjacent the sections shaped to form the sidewalls of the plurality of wells in a location corresponding to the bottom walls of the plurality of wells; (B) injecting molten light impermeable material into the mold cavity; and (C) cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells; wherein step (B) includes a step of injecting the molten light impermeable material into the mold cavity at a temperature and pressure sufficient to cause the light impermeable material to penetrate completely through the plate of light permeable material at predetermined locations. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification