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Nucleotide sequence detection with signal amplification

  • US 6,033,853 A
  • Filed: 01/08/1997
  • Issued: 03/07/2000
  • Est. Priority Date: 03/19/1996
  • Status: Expired due to Fees
First Claim
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1. A kit for the detection of a target nucleotide sequence with signal amplification, wherein, in appropriate containers, said kit comprises:

  • a) a nucleotide probe that is labeled with a marker, andb) a reagent comprising a suspension of insoluble particles wherein at least one series of oligonucleotide units is immobilized on said particles;

    wherein within each said series said oligonucleotide units are identical and comprise;

    a first nucleotide sequence that specifically hybridizes with said target nucleotide sequence, anda second nucleotide sequence that specifically hybridizes with said nucleotide probe; and

    wherein said reagent has more than 10 of said oligonucleotide units per particle.

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