Nucleotide sequence detection with signal amplification
First Claim
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1. A kit for the detection of a target nucleotide sequence with signal amplification, wherein, in appropriate containers, said kit comprises:
- a) a nucleotide probe that is labeled with a marker, andb) a reagent comprising a suspension of insoluble particles wherein at least one series of oligonucleotide units is immobilized on said particles;
wherein within each said series said oligonucleotide units are identical and comprise;
a first nucleotide sequence that specifically hybridizes with said target nucleotide sequence, anda second nucleotide sequence that specifically hybridizes with said nucleotide probe; and
wherein said reagent has more than 10 of said oligonucleotide units per particle.
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Abstract
Kit for detecting a specific target nucleic acid sequence using a labeled probe with signal amplification, which comprises in containers (i) a probe labeled with a tracer (ii) a suspension of insoluble particles on which are immobilized at least one series of oligonucleotide units all of which are identical and contain at least one sequence that specifically hybridize with the target nucleic acid sequence and at one sequence that specifically hybridize with tracer labeled probe. A method using the kit is also described.
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12 Claims
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1. A kit for the detection of a target nucleotide sequence with signal amplification, wherein, in appropriate containers, said kit comprises:
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a) a nucleotide probe that is labeled with a marker, and b) a reagent comprising a suspension of insoluble particles wherein at least one series of oligonucleotide units is immobilized on said particles;
wherein within each said series said oligonucleotide units are identical and comprise;a first nucleotide sequence that specifically hybridizes with said target nucleotide sequence, and a second nucleotide sequence that specifically hybridizes with said nucleotide probe; and wherein said reagent has more than 10 of said oligonucleotide units per particle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for detecting with signal amplification a target nucleotide sequence comprising adding to a liquid medium under conditions allowing hybridization, a nucleotide probe that is labeled with a marker, and a reagent comprising a suspension of insoluble particles wherein at least one series of oligonucleotide units is immobilized on said particles;
- wherein within each said series said oligonucleotide units are identical and comprise;
a first nucleotide sequence that specifically hybridizes with said target nucleotide sequence, and a second nucleotide sequence that specifically hybridizes with said nucleotide probe; wherein said reagent has more than 10 of said oligonucleotide units per particle; and wherein the presence of target nucleotide sequence is detected. - View Dependent Claims (10, 11, 12)
- wherein within each said series said oligonucleotide units are identical and comprise;
Specification