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Methods for obtaining nucleic acid containing a mutation

  • US 6,033,861 A
  • Filed: 11/18/1998
  • Issued: 03/07/2000
  • Est. Priority Date: 11/19/1997
  • Status: Expired due to Fees
First Claim
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1. A method for preparing a heteroduplex-enriched nucleic acid sample from an organism carrying heterozyygous mutations, comprising the steps of:

  • (a) denaturing substantially all of the double-stranded nucleic acid present in a nucleic acid sample from an organism;

    (b) allowing the nucleic acid to anneal the under conditions which permit formation of heteroduplexes and homoduplexes; and

    (c) removing homoduplexes from the annealed sample wherein the removal of said homoduplexes is effected using immobilized mismatch binding proteins (MBP), thereby retaining heteroduplexes in said sample.

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