Automated assaying device

• US 6,033,911 A
• Filed: 02/27/1998
• Issued: 03/07/2000
• Est. Priority Date: 02/27/1998
• Status: Expired due to Term

• Current Assignee: Hamilton Company
• Original Assignee: Hamilton Company
• Inventors: Johnson, James E., Gulla, Gregory, Schultz, Harold R.
• Primary Examiner(s): Le, Long V.
• Application Number:
  US09/032,741
• Time in Patent Office: 739 Days
• Field of Search: 436/43, 436/49, 436/54, 436/174, 436/180, 422/63, 422/65, 422/81, 422/100, 422/103, 422/104, 73/863.32, 73/863.24, 73/863.25, 141/1, 141/130, 141/180, 141/181, 141/237, 141/238
• US Class Current: 436/49
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An automated assaying system is disclosed having a multiplicity of lumens oriented and controllable in clusters. The lumens are portrayed in a matrix, wherein each row of the matrix consists of one such cluster that is individually controllable for aspiration and dispensation purposes. Also provided is a unique wash system capable of flushing the entirety of the system. A method is also depicted for accomplishing this unique assaying.

Claims

1. An automated assaying apparatus, comprising, in combination:

a plurality of lumens sequestered into independently fed clusters and ganged into a bundle;

three-dimensional moving means to orient said bundle;

fluid treatment means connected to said clusters to feed said clusters

wherein said fluid treatment means includes a fluid pump and a fluid source delivering fluid to said pump and a plurality of valves, one valve per said cluster, coupled between said clusters and said pump and each said valve operatively coupled to one syringe housing having a plurality of syringes therein.

2. The apparatus of claim 1 wherein said valve comprises:

a fluid port to receive fluid from said pump,

an outer sleeve which circumscribes said fluid port, and

an internal shaft residing within said outer sleeve and acting as a valve

key sleeve and having fluid transferring grooves which deliver or prevent fluid to pass into each said syringe in its respective said syringe housing via interposed outlet ports which are alternatively aligned or obstructed based on orientation of said grooves.

3. The apparatus of claim 2 wherein there are two said grooves, each of which feed four said syringes-through said outlet ports.

4. The apparatus of claim 3 wherein four said outlet ports are located above and four said outlet ports below said outer sleeve, said above outlet ports offset from said below outlet ports allowing 180°

rotation of said internal shaft for fluid admission/preclusion.

5. The automated assaying apparatus of claim 2 wherein said plurality of syringes are oriented one syringe per lumen.

6. The automated assaying apparatus of claim 5 wherein said fluid treatment means includes a first plurality of motors, one operatively coupled to each said valve, and a second plurality of motors, one operatively coupled to said plurality of syringes within said syring housing.

7. The automated assaying apparatus of claim 6 further comprising a plurality of probes removably coupled to an end of said lumens.

8. The automated assaying apparatus of claim 7 wherein said fluid treatment moans includes wash means for cleansing said lumens and probes.

9. The automated assaying apparatus of claim 8 further comprising lumen management means for flexibly containing said bundle.

10. The automated assaying apparatus of claim 9 further comprising swivel means coupled to said lumen management means for ensuring lumen integrity during movement by said three-dimensional moving means.

11. The automated assaying apparatus of claim 10 further comprising a plurality of probe receiving means for sequestering samples oriented in an operatively spatial relationship to said probes.

12. The automated assaying apparatus of claim 11 wherein said wash means includes a trough containing a plurality of probe receiving means and drainage means operatively coupled to said trough.

13. The automated assaying apparatus of claim 12 wherein said probes and said probe receiving means are oriented into complementary matrices.

14. A method for assaying fluids, the steps including:

placing a plurality of probes having a multiplicity of feed lines above a work surface;

sequestering the probes and feed lines into clusters

orienting a receiver on the work surface;

addressing the receiver with the probes;

independently treating the probes in clusters with fluid provided by the receiver;

loading the clusters of probes with fluid by interposing clusters of syringes between the probes and a fluid;

and valving fluid into the syringes in the clusters, one value per cluster.

15. The method of claim 14 wherein said valving includes:

fluid porting to receive fluid,

pumping fluid therethrough forming the valve as an outer sleeve which circumscribes the fluid porting, and

placing an internal shaft within said outer sleeve and acting as a valve key sleeve and having fluid transferring grooves which deliver or prevent fluid to pass into syringes in a respective syringe housing via interposing outlet ports and alternatively aligning or obstructing the outlets based on orientation of grooves formed in the sleeve.

16. The method of claim 15 further comprising the step of precluding feed line fouling.

17. The method of claim 16 further comprising the step of orienting the probes within a wash basin and washing the probes.

18. An automated assaying system, comprising, in combination:

a plurality of fluid treatment means oriented in a matrix;

said plurality of fluid treatment means oriented in clusters; and

means to provide fluid to independent clusters via a plurality of valves, one valve per cluster, wherein said valve comprises;

a fluid port to receive fluid from said pump,

an outer sleeve which circumscribes said fluid port, and

an internal shaft residing within said outer sleeve and acting as a valve

key sleeve and having fluid transferring grooves which deliver or prevent fluid to pass into a syringe in its respective syringe housing via interposed outlet ports which are alternatively aligned or obstructed based on orientation of said grooves.

19. The automated assaying system of claim 18 further comprising a complementarily formed matrix of wells oriented on a table top.

20. The automated assaying system of claim 19 wherein said plurality of syringes are oriented one per said fluid treatment means.

21. The automated assaying system of claim 20 wherein said matrix includes robotic means for moving said matrix in three dimensions above said table.

22. An automated assaying system, comprising, in combination:

a plurality of fluid treatment means oriented in a matrix;

said plurality of fluid treatment means oriented in clusters each said cluster controlled by one valve communicating with syringes in a syringe housing wherein said valve comprises;

a fluid port to receive fluid from said pump,

an outer sleeve which circumscribes said fluid port, and

an internal shaft residing within said outer sleeve and acting as a valve key sleeve and having fluid transferring grooves which deliver or prevent fluid to pass into each said syringe in its respective said syringe housing via interposed outlet ports which are alternatively aligned or obstructed based on orientation of said grooves; and

means to purge said fluid treatment means for subsequent reuse.

23. A fluid sampling matrix, comprising, in combination:

a robotic arm carrying said matrix;

said matrix coupled to a plurality of lumens ganged in clusters, each said cluster communicating with one syringe housing supporting a plurality of syringes, one per lumen, a valve per syringe housing operable to deliver or preclude fluid to each said syringe in said syringe housing, wherein said valve comprises;

a fluid port to receive fluid from said pump,

an outer sleeve which circumscribes said fluid port, and

an internal shaft residing within said outer sleeve and acting as a valve key sleeve and having fluid transferring grooves which deliver or prevent fluid to pass into each said syringe in its respective said syringe housing via interposed outlet ports which are alternatively aligned or obstructed based on orientation of said grooves;

means to address said matrix to a work surface; and

means to constrict rotation of the matrix vis-a-vis said work surface.