SH3 kinase domain associated protein, a signalling domain therein, nucleic acids encoding the protein and the domain, and diagnostic and therapeutic uses thereof

• US 6,034,212 A
• Filed: 06/07/1995
• Issued: 03/07/2000
• Est. Priority Date: 12/01/1994
• Status: Expired due to Fees

• Current Assignee: Max-Delbruck-Centrum Fur Molekulare Medizin, Rockefeller University
• Original Assignee: Max-Delbruck-Centrum Fur Molekulare Medizin, Rockefeller University
• Inventors: Chen, Henry, Bork, Peer, Sudol, Marius
• Primary Examiner(s): Priebe, Scott D.
• Assistant Examiner(s): Nguyen, Dave Trong
• Application Number:
  US08/476,509
• Time in Patent Office: 1,735 Days
• Field of Search: 435/194, 435/193, 530/350, 530/300
• US Class Current: 530/324
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/46 from vertebrates
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

The present invention relates to regulation and control of cellular processes by SH3-domain binding proteins, by putative signalling domains of such proteins, ligands of the signalling domain, and diagnosis and therapy based on the activity of such proteins, signalling domains, and ligands.

Claims

1. An isolated Yes proto-oncogene associated protein or polypeptide (YAP) that comprises the amino acid sequence of SEQ ID NO:

2.

2. An isolated Yes proto-oncogene associated protein or polypeptide (YAP) that comprises the amino acid sequence of SEQ ID NO:

4.

3. An isolated Yes proto-oncogene associated protein or polypeptide (YAP) that comprises the amino acid sequence of SEQ ID NO:

5.

4. A peptide consisting of 30 to 50 amino acid residues comprising a WW domain that comprises an amino acid sequence selected from the group consisting of SEQ ID NO:

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, SEQ ID NO;

19, SEQ ID NO;

20, SEQ ID NO;

21, SEQ ID NO;

22, SEQ ID NO;

23, SEQ ID NO;

24, and SEQ ID NO;

25.

5. The peptide of claim 4 that comprises the amino acid sequence of SEQ ID NO:

6.

6. The peptide of claim 4 that comprises the amino acid sequence of SEQ ID NO:

9.

7. The peptide of claim 4 that comprises the amino acid sequence of SEQ ID NO:

10.

8. The peptide of claim 4 that comprises the amino acid sequence of SEQ ID NO:

11.

9. The peptide of claim 4 that comprises the amino acid sequence of SEQ ID NO:

12.

10. The peptide of claim 4 which is labeled.

11. A chimeric polypeptide consisting of a protein and a peptide;

wherein the protein contains at least a portion of a non-YAP or non-WW domain protein; and

wherein the peptide consists of 30 to 50 amino acid residues; and

wherein said peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO;

6, SEQ ID NO;

7, SEQ ID NO;

8, SEQ ID NO;

9, SEQ ID NO;

10, SEQ ID NO;

11, SEQ ID NO;

12, SEQ ID NO;

13, SEQ ID NO;

14, SEQ ID NO;

15, SEQ ID NO;

16, SEQ ID NO;

17, SEQ ID NO;

18, SEQ ID NO;

19, SEQ ID NO;

20, SEQ ID NO;

21, SEQ ID NO;

22, SEQ ID NO;

23, SEQ ID NO;

24, and SEQ ID NO;

25.

12. The chimeric polypeptide of claim 11 wherein the protein is glutathione-S-transferase.