Wavelength-shifting probes and primers and their use in assays and kits

• US 6,037,130 A
• Filed: 07/28/1998
• Issued: 03/14/2000
• Est. Priority Date: 07/28/1998
• Status: Expired due to Term

• Current Assignee: The Public Health Research Institute of the City of New York, Inc.
• Original Assignee: Public Health Institute of The City of New York Incorporated
• Inventors: Marras, Salvatore A. E., Kramer, Fred R., Tyagi, Sanjay
• Primary Examiner(s): Marschel, Ardin H.
• Application Number:
  US09/123,764
• Time in Patent Office: 595 Days
• Field of Search: 435/6, 435/810, 435/91.1, 435/91.2, 436/501, 536/22.1, 536/23.1, 536/24.1-24.33, 536/25.3, 935/77, 935/78
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 2525/107 incorporating a peptide nucleic acid
• CPC Class Codes: C12Q 2525/301 Hairpin oligonucleotides
• CPC Class Codes: C12Q 2531/119 Strand displacement amplification [SDA]
• CPC Class Codes: C12Q 2531/137 Ligase Chain Reaction [LCR]
• CPC Class Codes: C12Q 2531/143 Promoter based amplification, e.g. NASBA, 3SR, TAS

Abstract

Hairpin-forming oligonucleotide probes and primers triple-labeled with a pair of florophores, a shorter wavelength harvester and a longer wavelength emitter, and a quencher. When the probes and primers are stimulated by light that excites the harvester, opening causes an increase in fluorescence by the emitter, while fluorescence from the harvester is continually suppressed. The probes and primers may be used for detection of nucleic acid targets in assays, including amplification assays. Assay kits are provided.

Claims

1. A fluorescently labeled hairpin-forming oligonucleotide containing:

a) a fluorescent emitter moiety having an excitation spectrum and an emission spectrum including a maximum emission wavelength;

b) a fluorescent harvester moiety having an excitation spectrum including a maximum excitation wavelength, having an emission spectrum that overlaps the excitation spectrum of the emitter moiety and including a maximum emission wavelength, the emission of the harvester moiety at its maximum emission wavelength having a first magnitude when said harvester moiety is unquenched and stimulated at its maximum excitation wavelength; and

c) a quencher moiety capable of quenching the fluorescence of at least one of the emitter moiety and the harvester moiety,

said oligonucleotide having a closed conformation including a single-stranded loop and a stem duplex, wherein said quencher moiety is in a quenching relationship to at least one of said harvester and emitter moieties and wherein, when excited at the maximum excitation wavelength of the harvester moiety, emission at the maximum emission wavelength of the harvester moiety is substantially suppressed relative to said first magnitude and emission at the maximum emission wavelength of the emitter moiety has a second magnitude, and

said oligonucleotide having an open conformation not including said stem duplex in which said quencher moiety is not in a quenching relationship with said harvester or said emitter moiety wherein, when excited at the maximum excitation wavelength of the harvester moiety, emission at the maximum emission wavelength of the harvester moiety is substantially suppressed relative to said first magnitude, energy is transferred from the harvester moiety to the emitter moiety, and emission at the maximum emission wavelength of the emitter moiety is detectably greater than said second magnitude.

2. The oligonucleotide of claim 1 wherein hybridization of said loop to a target sequence causes said oligonucleotide to assume its open conformation.

3. The oligonucleotide of claim 2 wherein said quencher moiety is selected from the group consisting of DABCYL and variants of DABCYL.

4. The oligonucleotide of claim 2 wherein, when said oligonucleotide is in its closed conformation, the emitter moiety is located on said oligonucleotide terminally to said stem duplex, the quencher and harvester moieties are located on said oligonucleotide in said stem duplex, and the harvester and quencher moieties are in a quenching relationship.

5. The oligonucleotide of claim 2 wherein, when said oligonucleotide is in its open conformation said emission at the maximum emission wavelength of the emitter is at least four times as great as said second magnitude.

6. The oligonucleotide of claim 2 wherein, when said oligonucleotide is in its closed conformation, said quencher and emitter moieties are located on said oligonucleotide in said stem duplex in a quenching relationship.

7. The oligonucleotide of claim 2 wherein at least one of said harvester and emitter moieties forms part of the nucleotide chain of said oligonucleotide.

8. The oligonucleotide of claim 2 wherein at least one of said harvester, emitter and quencher moieties is attached to said oligonucleotide through an alkyl linker.

9. The oligonucleotide of claim 2 wherein said oligonucleotide includes at least one of the group of modified nucleotides and modified linkages.

10. The oligonucleotide of claim 9 wherein said oligonucleotide comprises peptide nucleic acid (PNA).

11. The oligonucleotide of claim 9 wherein said oligonucleotide includes 2'"'"'-O-methylribonucleotides.

12. The oligonucleotide of claim 2 wherein one strand of said stem duplex is complementary to said target strand, whereby said oligonucleotide is capable of serving as a primer for a DNA polymerase.

13. The oligonucleotide of claim 1 wherein said oligonucleotide includes a terminal extension capable of serving as a priming region for a DNA polymerase when said oligonucleotide is in its closed conformation.

14. The oligonucleotide of claim 13 wherein said emitter moiety is located on said terminal extension and said quencher and harvester moieties are located on said oligonucleotide in said stem duplex.

15. The oligonucleotide of claim 13 wherein at least one of said harvester and emitter moieties forms part of the nucleotide chain of said oligonucleotide.

16. The oligonucleotide of claim 13 wherein at least one of said harvester, emitter and quencher moieties is attached to said oligonucleotide through an alkyl linker.

17. The oligonucleotide of claim 13 wherein said oligonucleotide includes at least one of the group of modified nucleotides and modified linkages.

18. A reagent kit comprising ingredients for a nucleic acid amplification, a detector probe that is an oligonucleotide according to claim 2, and instructions for carrying out said amplification reaction.

19. The kit of claim 18 wherein said amplification is selected from the group consisting of polymerase chain reaction (PCR), strand displacement amplification (SDA), transcription mediated amplification (TMA), ligase chain reaction (LCR), nucleic acid sequence based amplification (NASBA), rolling circle amplification, and amplification of RNA by an RNA-directed RNA polymerase.

20. The kit according to claim 18 wherein said oligonucleotide is designed to hybridize to a perfectly complementary target sequence but not to hybridize significantly to a sequence differing therefrom by at least one nucleotide.

21. The kit according to claim 18 wherein the emitter moiety is located on said oligonucleotide terminally to said stem duplex and the quencher and harvester moieties are located on said oligonucleotide in said stem duplex.

22. A reagent kit for an amplification reaction that includes at least one primer, comprising the ingredients for said amplification assay and instructions for carrying out said amplification assay, wherein said at least one primer is an oligonucleotide according to claim 13.

23. The kit according to claim 22 wherein said amplification reaction is selected from the group consisting of the polymerase chain reaction (PCR), strand displacement amplification (SDA), transcription mediated amplification (TMA), ligase chain reaction (LCR), nucleic acid sequence based amplification (NASBA), rolling circle amplification, and amplification of RNA by an RNA-directed RNA polymerase.

24. The kit according to claim 23 wherein said oligonucleotide includes 2'"'"'-O-methylribonucleotides.

25. A detection assay comprising adding to a sample that might contain a target strand at least one detector probe that is an oligonucleotide according to claim 2 and detecting fluorescence emission from said at least one probe'"'"'s emitter moiety.

26. The assay according to claim 25 further comprising detecting fluorescence emission from said at least one probe'"'"'s harvester moiety.

27. The assay according to claim 26 further comprising adding said at least one detector probe to a sample that does not contain said target strand and detecting fluorescence emission of said harvester and emitter moieties.

28. The assay according to claim 25 further comprising adding said detector probe to a sample that does not contain said target strand and detecting fluorescence emission of said at least one probe'"'"'s emitter moiety.

29. The assay according to claim 25 wherein the emitter moiety is of said at least one probe located on said oligonucleotide terminally to said stem duplex and the quencher and harvester moieties are located on said oligonucleotide in said stem duplex.

30. The assay according to claim 25 wherein one of the harvester and emitter moieties of said at least one probe is a phosphoramidite derivative of a fluorophore that is incorporated in said oligonucleotide.

31. The assay according to claim 25 wherein said at least one probe comprises at least two probes, each having a different emitter moiety.

32. The assay according to claim 25, wherein said assay is selected from the group consisting of in situ and in vivo assays.

33. An amplification assay comprising adding to a sample that might contain a target strand the reagents to perform an amplification reaction selected from the group consisting of the polymerase chain reaction (PCR), strand displacement amplification (SDA), transcription mediated amplification (TMA), ligase chain reaction (LCR), nucleic acid sequence based amplification (NASBA), rolling circle amplification, and amplification of RNA by an RNA-directed RNA polymerase, and at least one detector probe according to claim 2 and detecting fluorescence emission from said at least one probe'"'"'s emitter moiety.

34. The assay according to claim 33, wherein said assay is selected from the group consisting of in situ and in vivo assays.

35. The assay according to claim 33 further comprising detecting fluorescence emission from said at least one probe'"'"'s harvester moiety.

36. The assay according to claim 35 further comprising adding said at least one detector probe to a sample that does not contain said target strand and detecting fluorescence emission of said at least one probe'"'"'s harvester and emitter moieties.

37. The assay according to claim 33 further comprising adding said at least one detector probe to a sample that does not contain said target strand and detecting fluorescence emission of said at least one probe'"'"'s emitter moiety.

38. The assay according to claim 33 wherein the emitter moiety is located on said oligonucleotide terminally to said stem duplex and the quencher and harvester moieties are located on said oligonucleotide in said stem duplex.

39. The assay according to claim 33 wherein one of the harvester and emitter moieties of said at least one probe is a phosphoramidite derivative of a fluorophore that is incorporated in said oligonucleotide.

40. The assay according to claim 33 wherein said at least one probe comprises at least two probes, each having a different emitter moiety.

41. The assay according to claim 33 wherein said oligoncleotide includes 2'"'"'-O-methylribonucleotides.

42. An amplification assay comprising an amplification reaction that includes at least one primer comprising the steps of adding to a sample that might contain a target strand the reagents to perform said amplification reaction, said reagents including at least one primer according to claim 12, and detecting fluorescence of said emitter moiety.

43. The assay according to claim 42, wherein said assay is selected from the group consisting of in situ and in vivo assays.

44. The amplification assay according to claim 42 wherein said at least one primer according to claim 12 comprises at least two primers.

45. An amplification assay comprising an amplification reaction that includes at least one primer comprising the steps of adding to a sample that might contain a target strand the reagents to perform said amplification reaction, said reagents including at least one primer according to claim 13, and detecting fluorescence of said emitter moiety.

46. The amplification assay according to claim 45 wherein said at least one primer according to claim 13 comprises at least two primers.

47. The amplification assay according to claim 45 further comprising detecting fluorescence emission from said harvester moiety.

48. The amplification assay according to claim 45 wherein the emitter moiety is located on said oligonucleotide terminally to said stem duplex and the quencher and harvester moieties are located on said oligonucleotide in said stem duplex.

49. The assay according to claim 45 wherein one of the harvester and emitter moieties of said at least one probe is a phosphoramidite derivative of a fluorophore that is incorporated in said oligonucleotide.

50. The assay according to claim 45 wherein said at least one probe comprises at least two probes, each having a different emitter moiety.