Culture compression device

• US 6,037,141 A
• Filed: 06/04/1999
• Issued: 03/14/2000
• Est. Priority Date: 06/04/1998
• Status: Expired due to Term

• Current Assignee: Flexcell International Corporation (Global PVQ SE)
• Original Assignee: Albert J. Banes
• Inventors: Banes, Albert J.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/326,396
• Time in Patent Office: 284 Days
• Field of Search: 435/395, 435/401, 435/420, 435/286.6, 435/287.1, 435/288.3, 435/288.4, 435/289.1, 435/305.1, 435/305.2, 435/305.3, 435/305.4, 435/1.1, 435/29, 435/30, 73/760, 73/788, 73/794, 73/795, 73/796, 73/797, 73/818, 73/819, 73/831, 73/849, 73/856
• US Class Current: 435/30
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12Q 1/24 Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Abstract

A culture compression device including an enclosure having a wall and a base made from a flexible membrane. A sample support member is positioned on the membrane and retains a cell culture thereon. A stop member is mounted on top of the wall in overlying spaced apart relationship to the cell culture support. When the membrane flexes towards the stop member, the cell culture is compressed against the stop member.

Claims

1. A cell culture compression assembly comprising:

an enclosure comprising a wall and a base, said base comprising a flexible membrane;

a sample support member positioned on said membrane; and

a stop member mounted on said wall in overlying spaced apart relationship to said sample support member, wherein said membrane is configured to flex towards said stop member thereby compressing said sample support member against said stop member.

2. The cell culture compression assembly as claimed in claim 1 wherein said stop member comprises a flange surrounding a main portion, said flange being seated on said wall.

3. The assembly as claimed in claim 2 wherein said stop member main portion includes a lower surface, wherein a position of said lower surface is adjustable.

4. The assembly as claimed in claim 3 wherein said stop member main portion defines a hole and further comprises an insert, wherein said insert includes said lower surface and is movable through said hole.

5. The assembly as claimed in claim 4 wherein said insert is threadable into said hole.

6. The assembly as claimed in claim 1 wherein said stop member main portion defines a plurality of apertures, whereby when said membrane flexes towards said stop member, fluid present in said enclosure between said membrane and said stop member flows out of said enclosure through said apertures.

7. The assembly as claimed in claim 2 wherein said stop member main portion is made from a transparent material such that a cell culture present on the sample support member is visible through the main portion.

8. The assembly as claimed in claim 1 wherein said sample support member comprises a body and a retaining member fixed to a surface of said body, said retaining member defining an opening.

9. The assembly as claimed in claim 8 further comprising a sample holder removably positioned within said retaining member opening.

10. The assembly as claimed in claim 9 wherein said sample holder comprises a cell culture sheet and a holder body fixed to one side of said sheet, said holder body defining at least one sample chamber, the other side of said sheet being removably positioned on said body in said opening of said retaining member.

11. The assembly as claimed in claim 10 wherein said retaining member comprises a first ring and said holder body comprises a second ring.

12. The assembly as claimed in claim 10 wherein said holder body comprises a disk-shaped member defining a plurality of sample chambers.

13. The assembly as claimed in claim 8 wherein said retaining member is compressible to a height of about five millimeters above said surface of said body.

14. A cell culture compression assembly comprising:

a cell culture plate defining an opening and having a flexible membrane covering said opening;

a sample support member positioned on said membrane; and

a stop member positioned in overlying spaced apart relationship to said sample support member, said membrane being configured to flex towards said stop member, thereby compressing said sample support member against said stop member.

15. The assembly as claimed in claim 14 wherein said cell culture plate includes a wall surrounding said membrane thereby defining a cell culture well, and wherein said stop member comprises a flange portion and a main portion, said flange portion being seated on said wall.

16. The assembly as claimed in claim 15 wherein said stop member main portion extends into said well.

17. The assembly as claimed in claim 15 wherein said stop member main portion includes a lower surface, wherein a position of said lower surface is adjustable.

18. The assembly as claimed in claim 15 further comprising means for clamping said stop member against said walls.

19. The assembly as claimed in claim 15 wherein said cell culture plate defines a plurality of openings, each said opening being covered by said flexible membrane and being surrounded by a wall thereby defining a plurality of cell culture wells, said assembly further comprising a plurality of said stop members, each said stop member being seated on one of said walls.

20. The assembly as claimed in claim 15 wherein said stop member main portion is made from a transparent material such that a cell culture present on the sample support member is visible through the main portion.

21. The assembly as claimed in claim 15 wherein said main portion defines an opening and said stop member further comprises a transparent member covering said main portion opening such that said cell culture portion is visible through the main portion opening.

22. The assembly as claimed in claim 21 wherein said wall comprises a lower member and an upper member, said membrane being fixed between the lower member and the upper member and said flange portion of said stop member being fixed to the upper member.

23. A method of applying compressive forces to cultured cells comprising the steps of:

a) providing a flexible membrane;

b) positioning a sample support member containing a cell culture on the flexible membrane;

c) positioning a stop member in overlying spaced apart relationship to the sample support member; and

d) flexing the membrane in the direction of the stop member thereby compressing the cell culture against the stop member.

24. The method as claimed in claim 23 further comprising the steps of:

e) allowing the membrane to relax; and

f) repeating steps d) and e).

25. The method as claimed in claim 24 wherein the sample support member comprises a body and a retaining member fixed to one side of the body, such that the retaining member contains the cell culture, and wherein said step of flexing the membrane compresses the retaining member to a height of about five millimeters above the surface of the body.

26. The method as claimed in claim 25 wherein the membrane is incorporated into a cell culture plate defining an opening with the membrane covering the cell opening, the opening surrounded by a wall thereby defining a well, and wherein the stop member is positioned on the wall.

27. The method as claimed in claim 26 wherein the stop member extends into the well at an adjustable distance, such that the pressure exerted on the cell culture during said step of flexing the membrane is variable.