Scanning computed confocal imager

• US 6,038,067 A
• Filed: 02/06/1998
• Issued: 03/14/2000
• Est. Priority Date: 05/23/1996
• Status: Expired due to Fees

• Current Assignee: Los Alamos National Security LLC (Government of the United States of America)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: George, John S.
• Primary Examiner(s): Nguyen, Thong
• Assistant Examiner(s): Curtis, Craig
• Application Number:
  US09/019,863
• Time in Patent Office: 767 Days
• Field of Search: 359/618, 359/619, 359/237, 359/368
• US Class Current: 359/368
• CPC Class Codes: G02B 21/0028 specially adapted for specific applications, e.g. for endoscopes, ophthalmoscopes, attachments to conventional microscopes
• CPC Class Codes: G02B 21/004 fixed arrays, e.g. switchable aperture arrays
• CPC Class Codes: G02B 21/0056 based on optical coherence, e.g. phase-contrast arrangements, interference arrangements
• CPC Class Codes: G02B 21/0076 arrangements using fluorescence or luminescence

Abstract

There is provided a confocal imager comprising a light source emitting a light, with a light modulator in optical communication with the light source for varying the spatial and temporal pattern of the light. A beam splitter receives the scanned light and direct the scanned light onto a target and pass light reflected from the target to a video capturing device for receiving the reflected light and transferring a digital image of the reflected light to a computer for creating a virtual aperture and outputting the digital image. In a transmissive mode of operation the invention omits the beam splitter means and captures light passed through the target.

Claims

1. A confocal imager comprising:

a light source emitting light;

light modulating means in optical communication with said light source for producing a spatially and temporally varying pattern of illumination consisting of a plurality of discrete illumination sites;

beam splitting means receiving said spatially and temporally varying pattern of illumination for directing said spatially and temporally varying pattern of illumination through imaging optics onto a sample and passing reflected and fluorescent emission light from said sample after return passage through said imaging optics and said beam splitting means;

video capturing means receiving said reflected and fluorescent emission light from said imaging optics through said beam splitting means for acquiring a digital image of said reflected and fluorescent emission light and outputting said digital image; and

computer means receiving said digital image of said reflected and fluorescent emission light for creating a virtual aperture and synthesizing a composite confocal image of said sample and displaying said confocal image.

2. The confocal imager as described in claim 1 wherein said light modulating means comprises a liquid crystal display.

3. The confocal imager as described in claim 1 wherein said light modulating means comprises a ferroelectric liquid crystal.

4. The confocal imager as described in claim 1 wherein said light modulating means comprises a micro-mirror device.

5. The confocal imager as described in claim 1 wherein said light modulating means comprises an acousto-optic beam steering device.

6. The confocal imager as described in claim 1 wherein said light modulating means comprises an electromechanical beam scanning system.

7. The confocal imager as described in claim 1 wherein said light modulating means comprises a microlens array translation system.

8. The confocal imager as described in claim 1 wherein said video capturing means comprises a charge coupled device.

9. The confocal imager as described in claim 1 wherein said video capturing means comprises a charge injection device.

10. The confocal imager as described in claim 1 wherein said video capturing means comprises a photodiode camera.

11. The confocal imager as described in claim 1 wherein said imaging optics comprise a microscope objective.

12. The confocal imager as described in claim 1 wherein said imaging optics comprise a photographic lens.

13. The confocal imager as described in claim 1 wherein said imaging optics comprise a videographic lens.

14. The confocal imager as described in claim 1 wherein said imaging optics comprise a gradient index lens.

28. The confocal imager as described in claim 1, applied as a microscope or endoscope operating in the reflected light or fluorescence light modes, said confocal imager further comprising:

spectrally dispersive means receiving said reflected and fluorescent emission light from said imaging optics for dispersing said reflected and fluorescent emission light from each of said plurality of discrete illumination sites into a linear spectrum to form a spectrally resolved image;

said video capturing means receiving said spectrally resolved image and creating a digital image of said spectrally resolved image and outputting said digital image;

said computer means receiving said digital image of said spectrally resolved image for creating a virtual aperture and synthesizing a composite confocal spectrally resolved image of said sample; and

displaying said composite confocal spectrally resolved image.

15. A confocal image r comprising:

a light source emitting light;

light modulating means in optical communication with said light source for producing-a spatially and temporally varying pattern of illumination consisting of a plurality of discrete illumination sites;

first light focusing means receiving said spatially and temporally varying pattern of illumination for focusing said spatially and temporally varying pattern of illumination to scan across a sample;

second light focusing means for receiving light transmitted through said sample and focusing said transmitted light on video capturing means for receiving said transmitted light and outputting a digital image of said transmitted light;

computer means receiving said digital image of said transmitted light for creating a virtual aperture and synthesizing a confocal image of said sample and displaying said confocal image.

16. The confocal imager as described in claim 15 wherein said light modulating means comprises a liquid crystal display.

17. The confocal imager as described in claim 15 wherein said light modulating means comprises a ferroelectric liquid crystal.

18. The confocal imager as described in claim 15 wherein said light modulating means comprises a micro-mirror device.

19. The confocal imager as described in claim 15 wherein said light modulating means comprises a microlens array translation system.

20. The confocal imager as described in claim 15 wherein said light modulating means comprises an acousto-optic beam steering device.

21. The confocal imager as described in claim 15 wherein said light modulating means comprises an electromechanical beam scanning system.

22. The confocal imager as described in claim 15 wherein said video capturing means comprises a charge coupled device.

23. The confocal imager as described in claim 15 wherein said video capturing means comprises a photodiode array camera.

24. The confocal imager as described in claim 15 wherein said imaging optics comprise a microscope objective.

25. The confocal imager as described in claim 15 wherein said imaging optics comprise a photographic lens.

26. The confocal imager as described in claim 15 wherein said imaging optics comprise a videographic lens.

27. The confocal imager as described in claim 15 wherein said imaging optics comprise a gradient index lens.

29. The confocal imager as described in claim 15, applied as a microscope operating in the transmitted light mode, said confocal imager further comprising:

spectrally dispersive means receiving said transmitted light from said light modulating means for dispersing said transmitted light from each of said discrete illumination sites into a linear spectrum to form a spectrally resolved image;

said video capturing means receiving said spectrally resolved image and creating a digital image of said spectrally resolved image and outputting said digital image; and

said computer means receiving said digital image of said spectrally resolved image for creating a virtual aperture and synthesizing a composite confocal spectrally resolved image of said sample; and

displaying said composite confocal spectrally resolved image.