Defective adenoviruses and corresponding complementation lines
First Claim
1. A complementation line comprising a complementation element for complementing in trans a defective adenoviral vector,wherein said complementation element is either integrated into the chromosomal genome of said complementation line or inserted into an expression vector and said complementation element comprises a fragment of an adenoviral genome lacking an adenoviral 5'"'"'ITR and encoding an E1A gene product,wherein the E1A gene product is expressed under control of:
- a) an E1 promoter or a heterologous promoter when the complementation line is made from a human embryonic retinal cell or A549 cell;
b) an E1 promoter or a heterologous promoter when the complementation line is made by cotransfection of the expression vector comprising the complementation element and the defective adenoviral vector;
orc) an inducible heterologous promoter.
1 Assignment
0 Petitions
Accused Products
Abstract
Novel defective adenoviruses for the transfer and expression of an exogenous nucleotide sequence in a host cell or organism. The invention also relates to novel complementation lines and to the process for the preparation of these novel defective adenoviruses and their use in therapy and to a pharmaceutical composition containing same.
154 Citations
19 Claims
-
1. A complementation line comprising a complementation element for complementing in trans a defective adenoviral vector,
wherein said complementation element is either integrated into the chromosomal genome of said complementation line or inserted into an expression vector and said complementation element comprises a fragment of an adenoviral genome lacking an adenoviral 5'"'"'ITR and encoding an E1A gene product, wherein the E1A gene product is expressed under control of: -
a) an E1 promoter or a heterologous promoter when the complementation line is made from a human embryonic retinal cell or A549 cell; b) an E1 promoter or a heterologous promoter when the complementation line is made by cotransfection of the expression vector comprising the complementation element and the defective adenoviral vector;
orc) an inducible heterologous promoter. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A complementation line comprising a complementation element for complementing in trans a defective adenoviral vector,
wherein said complementation element is either integrated into the chromosomal genome of said complementation line or inserted into an expression vector and said complementation element comprises a fragment of an adenoviral genome lacking an adenoviral 5'"'"'ITR and encoding an E1A gene product, wherein the E1A gene product is expressed under control of: -
a) an E1 promoter or a heterologous promoter when the complementation line is made from a human embryonic retinal cell or A549 cell;
orb) an E1 promoter or a heterologous promoter when the complementation line is made by cotransfection of the expression vector comprising the complementation element and the defective adenoviral vector.
-
Specification