Method and means for magnetic particle specific binding assay

• US 6,040,192 A
• Filed: 08/26/1997
• Issued: 03/21/2000
• Est. Priority Date: 02/01/1993
• Status: Expired due to Term

• Current Assignee: Labsystems Oy (Thermo Fisher Scientific Incorporated)
• Original Assignee: Labsystems Oy (Thermo Fisher Scientific Incorporated)
• Inventors: Tuunanen, Jukka
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US08/920,094
• Time in Patent Office: 938 Days
• Field of Search: 436/526, 436/806, 436/808, 436/177, 422/101, 422/65, 210/222, 210/695, 209/212, 209/213, 209/214, 209/215, 209/217, 209/225, 435/287.1, 435/287.2, 435/287.3, 435/287.9
• US Class Current: 436/177
• CPC Class Codes: B01L 2200/0657 Pipetting powder
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0848 Specific forms of parts of containers
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 3/02 Burettes; Pipettes
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: B03C 1/286 disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: G01N 2035/1055 for immobilising reagents, e.g. dried reagents
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: G01N 35/109 with two horizontal degrees of freedom
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A system and method for handling magnetic particles, as in an immunoassay. A remover having a magnetic body and covering sleeve is inserted in a vessel containing magnetic particles and a liquid. The remover is moved up and down with the particles adhered to agitate the liquid and improve adherence of the particles.

Claims

1. A method for determining the presence of an analyte in a sample, the method comprising the steps of:

a. inserting a sample to be tested into a reaction vessel;

b. inserting solid magnetic particles coated with separating reagent into said reaction vessel to react with the sample;

c. removing said particles from said reaction vessel by inserting a magnet-equipped remover into said reacting vessel, wherein said particles adhere to said remover;

d. inserting the remover containing the particles into a measuring vessel wherein at least one of said reaction and measuring vessels contains a medium and agitating the medium by moving said remover vertically while said particles adhere to said remover to improve adherence of said particles;

e. measuring the reaction between the separating reagent and the sample; and

f. determining the presence of the analyte.

2. The method of claim 1 further comprising the steps of:

inserting the remover containing the particles into at least a third medium-containing vessel, agitating the medium in said third vessel and removing the particles from said third vessel.

3. The method of claim 1 wherein said steps include equipping said remover with a cover and reciprocating said cover-equipped remover.

4. The method of claim 1 wherein said reaction vessel contains the medium and a substance is attached to the medium in said reaction vessel.

5. The method of claim 4 wherein the sample reacts with the substance forming a reacted substance which binds any substance from the sample which may disturb later determining steps.

8. The method of claim 1 or 6 wherein the remover includes a sheath and a magnet insertable therein.

9. The method of claim 8 wherein the sheath and magnet are simultaneously inserted into said vessel.

6. A method for removing magnetic separating particles from a fluid, comprising:

providing a magnetic remover and a vessel containing magnetic particles and said fluid, said remover and said vessel shaped and sized such that vertical insertion of said remover into said vessel causes substantial fluid flow vertically between an inner wall of said vessel and said remover,

removing said magnetic particles from said vessel by inserting said remover into said vessel,

agitating said fluid by moving said remover up and down while said particles adhere to said remover, and

raising said remover from said vessel.

7. The method of claim 6 comprising agitation by repeatedly moving said remover up and down about 20 times in 10 seconds.

10. An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising:

a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample;

b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample;

c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample;

d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel;

e. a magnet-equipped remover for inserting and moving the particles from one vessel to another, said remover being vertically movable for agitating a medium contained in each of said vessels while said particles adhere to said remover to improve adherence of said particles.

11. The apparatus of claim 10 wherein the remover is equipped with an outer surface.

12. The apparatus of claim 11 wherein said outer surface has a profiled area for making the particles adhere to the remover as a dense mass.

13. The apparatus of claim 11 wherein said outer surface slopes downward.

14. The apparatus of claim 13 wherein said outer surface has a sharp nodule at its lowest point.

15. The apparatus of claim 10 wherein each of said vessels are joined together for form a single vessel unit.

16. The apparatus of claim 10 wherein at least one of said vessels is closed with a penetrable film.

17. The apparatus of claim 16 wherein each said vessel is closed with a penetrable film.

18. The apparatus of claim 16 wherein at least one closed vessel contains an inert gas.

20. The apparatus of claim 10 or 19 wherein the remover includes a sheath and a magnet insertable therein.

21. The apparatus of claim 20 wherein the sheath and magnet are simultaneously inserted into said vessel.

19. An apparatus for use in determining the presence of an analyte in a sample, the apparatus comprising:

a. a reaction vessel containing a sample to be analyzed and solid magnetic particles coated with a separating reagent, wherein the separating reagent reacts with the sample;

b. a plurality of vessels for performing intermediate steps on the particles after the reaction between the separating reagent and the sample;

c. a measuring vessel for conducting measurements of the reaction between the separating reagent and the sample;

d. a measuring device for measuring the reaction between the separating reagent and the sample in the measuring vessel;

e. a remover for inserting, removing and moving the particles from one vessel to another, said remover containing a magnet for making said particles adhere to said remover after the reaction, said remover being equipped with an outer surface, said outer surface having a profiled area for making said particles adhere to said remover as a dense mass, said outer surface sloping downwardly, and said outer surface being a sharp nodule at its lowest point, wherein at least one vessel contains a medium, and said remover being vertically movable for agitating the medium while said particles adhere to said remover to improve adherence of said particles.