Process for processing organic specimens
First Claim
1. A process for processing tissue comprising the steps of(i) fixing an organic sample to be processed,(ii) dehydrating the sample with a dehydration agent,(iii) clearing the sample with an essentially lipohilic agent, and(iv) impregnating the sample,wherein said steps (ii) and (iii) are carried out simultaneously by applying a mixture of said dehydration agent and said essentially lipophilic agent simultaneously to the sample.
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Abstract
This invention relates to the processing of organic tissues to prepare them for further investigation such as by light microscopy, electron microscopy, microwave staining, immunostaining, decalcification, hybridisation etc.
The present invention discloses a process for processing organic tissue, which comprises the steps of fixing an organic sample to be processed, dehydrating and clearing the sample and subsequently infiltrating and embedding the sample in a supporting medium to prepare it for further examination wherein the steps of dehydrating and clearing are carried out simultaneously by applying a mixture of a dehydration agent and an essentially lipophilic agent simultaneously to the sample.
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Citations
49 Claims
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1. A process for processing tissue comprising the steps of
(i) fixing an organic sample to be processed, (ii) dehydrating the sample with a dehydration agent, (iii) clearing the sample with an essentially lipohilic agent, and (iv) impregnating the sample, wherein said steps (ii) and (iii) are carried out simultaneously by applying a mixture of said dehydration agent and said essentially lipophilic agent simultaneously to the sample.
Specification