Integrated nucleic acid diagnostic device
First Claim
Patent Images
1. A miniature analytical device, comprising:
- a body having at least first, second and third chambers in fluid communication within said body;
said first chamber having disposed therein at least one reagent for amplifying a nucleic acid segment within a sample,said second chamber for fragmenting said nucleic acid segmentand said third chamber having an array of nucleic acid probes disposed therein, said array including a plurality of different nucleic acid sequences coupled to a surface of a single substrate, each of said plurality of different nucleic acid sequences being coupled to said surface in a different, known location.
5 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a miniaturized integrated nucleic acid diagnostic device and system. The device of the invention is generally capable of performing one or more sample acquisition and preparation operations, in combination with one or more sample analysis operations. For example, the device can integrate several or all of the operations involved in sample acquisition and storage, sample preparation and sample analysis, within a single integrated unit. The device is useful in a variety of applications, and most notably, nucleic acid based diagnostic applications and de novo sequencing applications.
691 Citations
37 Claims
-
1. A miniature analytical device, comprising:
-
a body having at least first, second and third chambers in fluid communication within said body; said first chamber having disposed therein at least one reagent for amplifying a nucleic acid segment within a sample, said second chamber for fragmenting said nucleic acid segment and said third chamber having an array of nucleic acid probes disposed therein, said array including a plurality of different nucleic acid sequences coupled to a surface of a single substrate, each of said plurality of different nucleic acid sequences being coupled to said surface in a different, known location. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
-
15. A miniature analytical device, comprising:
-
a body having at least three eeation chambers disposed therein, wherein each of said reaction chambers is fluidly connected to at least one other of said reaction chambers; a first of said reaction chambers for disrupting biological materials in a fluid sample; a second of said reaction chambers for fragmenting a nucleic acid; a third of said reaction chambers having a nucleic acid probe array disposed therein, said nucleic acid probe array comprising a plurality of different nucleic acid sequences coupled to a surface of a single substrate, each of said plurality of different nucleic acid sequences being coupled to said surface in a different, known location. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
-
-
26. A method of analyzing a nucleic acid test sample in a miniature analytic device having first, second and third chambers in fluid communication, comprising:
-
amplifying the nucleic acid test sample in a first chamber; transporting the nucleic acid test sample from the first chamber to a second chamber; fragmenting the nucleic acid test sample in the second chamber; transporting the nucleic acid test sample from the second chamber to a third chamber; and hybridizing the nucleic acid test sample to a nucleic acid probe under hybridization conditions in the third chamber. - View Dependent Claims (27, 28, 29, 30)
-
-
31. A method of using a miniature analytic device having first, second and third chambers in fluid communication to analyze a nucleic acid test sample contained in a biological material, comprising:
-
disrupting the biological material to extract the nucleic acid test sample in a first chamber; transporting the nucleic acid test sample from the first chamber to a second chamber; fragmenting the nucleic acid in the second chamber; transporting the nucleic acid test sample from the second chamber to a third chamber; and hybridizing the nucleic acid test sample to a nucleic acid probe under hybridization conditions in the third chamber. - View Dependent Claims (32, 33, 34, 35, 36, 37)
-
Specification