Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
First Claim
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1. A solid phase support comprising:
- (a) a transponder which is a monolithic integrated circuit which can transmit via electromagnetic radiation data stored within said integrated circuit; and
(b) an oligonucleotide probe attached to the surface of the integrated circuit, wherein said probe is capable of binding to a nucleic acid.
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Abstract
A method is described for determining the sequence of nucleic acids. The method employs small solid phase particles having transponders, with a primary layer of an oligonucleotide of known sequence attached to the outer surface of the particle. A read/write scanner device is used to encode and decode data on the transponder. The stored data includes the sequence of the oligonucleotide immobilized on the transponder. The sequence of sample nucleic acids is determined by detecting annealing to an oligonucleotide bound to a particle, followed by decoding the transponder to determine the sequence of the oligonucleotide.
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Citations
16 Claims
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1. A solid phase support comprising:
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(a) a transponder which is a monolithic integrated circuit which can transmit via electromagnetic radiation data stored within said integrated circuit; and (b) an oligonucleotide probe attached to the surface of the integrated circuit, wherein said probe is capable of binding to a nucleic acid. - View Dependent Claims (3, 4, 5)
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2. A solid phase support comprising:
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(a) a transponder which is a monolithic integrated circuit which can transmit via electromagnetic radiation data stored within said integrated circuit; (b) at least one layer of a coating deposited on the surface of the integrated circuit, (c) an oligonucleotide probe attached to the coating layer, wherein said probe is capable of binding to a target nucleic acid. - View Dependent Claims (6, 7, 8)
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9. A kit for determining the sequence of target nucleic acids in a sample, comprising:
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(a) at least one assay vessel, containing at least one solid phase particle having (i) a transponder and (ii) an oligonucleotide probe bound to its surface; and (b) at least one label reagent. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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Specification