High throughput screening assay systems in microscale fluidic devices
First Claim
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1. An apparatus for screening test compounds for an effect on a flowing biochemical system, comprising:
- a substrate having at least one surface;
at least two intersecting channels fabricated into said surface of said substrate, at least one of said at least two intersecting channels having at least one cross-sectional dimension in the range from about 0.1 to about 500 μ
m;
a source of a plurality of different test compounds fluidly connected to a first of said at least two intersecting channels;
a source of at least one component of said biochemical system fluidly connected to a second of said at least two intersecting channels;
a fluid direction system for flowing said at least one component within said second of said at least two intersecting channels and for flowing said different test compounds from said first to said second of said at least two intersecting channels;
a cover mated with said surface; and
a detection zone in said second channel for detecting an effect of said test compound on said flowing biochemical system.
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
923 Citations
38 Claims
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1. An apparatus for screening test compounds for an effect on a flowing biochemical system, comprising:
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a substrate having at least one surface; at least two intersecting channels fabricated into said surface of said substrate, at least one of said at least two intersecting channels having at least one cross-sectional dimension in the range from about 0.1 to about 500 μ
m;a source of a plurality of different test compounds fluidly connected to a first of said at least two intersecting channels; a source of at least one component of said biochemical system fluidly connected to a second of said at least two intersecting channels; a fluid direction system for flowing said at least one component within said second of said at least two intersecting channels and for flowing said different test compounds from said first to said second of said at least two intersecting channels; a cover mated with said surface; and a detection zone in said second channel for detecting an effect of said test compound on said flowing biochemical system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. An apparatus for detecting an effect of a test compound on a flowing biochemical system, comprising:
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a substrate having at least one surface; a plurality of reaction channels fabricated into said surface; at least two transverse channels fabricated into said surface, each of said plurality of reaction channels being fluidly connected to a first of said at least two transverse channels at a first point in said reaction channels, and fluidly connected to a second of said at least two transverse channels at a second point in said reaction channels, said at least two transverse channels and said plurality of reaction channels each having at least one cross-sectional dimension in the range from about 0.1 to about 500 μ
m;a source of at least one component of said biochemical system, said source of at least one component of said biochemical system being fluidly connected to each of said plurality of reaction channels; a source of test compounds fluidly connected to said first of said at least two transverse channels; a fluid direction system for controlling movement of said test compound and said at least one component within said at least two transverse channels and said plurality of reaction channels; a cover mated with said surface; and a detection system for detecting an effect of said test compound on said flowing biochemical system. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification