Microfluidic devices and systems

• US 6,048,498 A
• Filed: 11/12/1998
• Issued: 04/11/2000
• Est. Priority Date: 08/05/1997
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Kennedy, Colin B.
• Primary Examiner(s): Gulakowski, Randy
• Assistant Examiner(s): CARRILLO, BIBI SHARIDAN
• Application Number:
  US09/190,428
• Time in Patent Office: 516 Days
• Field of Search: 422/55, 422/68.1, 422/58, 422/82.05, 422/99, 422/104, 422/100, 422/108, 204/297 R, 204/601, 204/603, 204/451, 204/452, 73/864.91, 356/244, 356/246, 220/755, 220/771, 220/345, 215/395, 206/261, 206/263
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/087 Ergonomic aspects
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

Microfluidic apparatus which include a planar device and polymeric holder assembly are provided. The polymeric holder assembly includes a cavity disposed through the holder, providing optical access to the top and bottom of the planar device when the device is mounted in the polymeric holder. The planar device includes microscale channels disposed within the device.

Claims

1. A microfluidic apparatus, comprising:

a planar microfluidic device comprising a top planar surface and a bottom planar surface, the planar microfluidic device having disposed therein at least two intersecting channels, the channel structure falling within an area of the planar microfluidic device of less than 6 cm², and wherein the top surface of the microfluidic device comprises a plurality of wells disposed therein, wherein said wells are in fluid communication with at least one of said at least two intersecting channels; and

,

a polymeric holder assembly comprising an additional surface which comprises a cavity disposed therethrough, the cavity being configured to fixedly receive the microfluidic device, wherein the cavity comprises an aperture disposed through the polymeric holder assembly, said aperture providing optical access to the top and bottom surfaces of the microfluidic device, the additional surface extending from at least one edge of the cavity.

2. The microfluidic apparatus of claim 1, wherein the channel structure falls within an area of the planar microfluidic device of less than 5 cm².

3. The microfluidic apparatus of claim 1, wherein the channel structure falls within an area of the planar microfluidic device of less than 4 cm².

4. The microfluidic apparatus of claim 1, wherein the channel structure falls within an area of the planar microfluidic device of less than 3 cm².

5. The microfluidic apparatus of claim 1, wherein the channel structure falls within an area of the planar microfluidic device of less than 2 cm².

6. The microfluidic apparatus of claim 1, wherein the planar microfluidic device comprises dimensions that are less than about 2 cm by less than about 2 cm.

7. The microfluidic apparatus of claim 1, wherein the planar microfluidic device comprises dimensions that are less than about 1.5 cm by less than about 1.5 cm.

8. The microfluidic apparatus of claim 1, wherein the aperture provides optical access to at least one of the two intersecting channels in the microfluidic device, when the micro fluidic device is inserted into the cavity.

9. The microfluidic device of claim 1, wherein the additional surface comprises a plurality of raised ridges disposed thereon.

10. The microfluidic device of claim 1, wherein a side edge of the polymeric holder assembly comprises a beveled edge.

11. The microfluidic device of claim 10, wherein the beveled edge of the polymeric holder assembly extends only part way up the side edge.

12. A microfluidic apparatus, comprising:

a first polymeric substrate layer having a plurality of grooves fabricated into a first surface thereof;

a second polymeric substrate layer mated with the first surface of the first polymeric substrate layer, the second polymeric substrate layer being bonded to the first surface of the first polymeric substrate layer, thereby defining microscale channel network between the first and second polymeric substrate layers, the microscale channel network falling within an area of the first surface of the first polymeric substrate layer of less than 6 cm² ; and

wherein at least one of the first and second polymeric substrate layers extends disproportionately in one dimension from the an area where the first and second polymeric substrate layers are bonded together in which the microscale channel network falls, to provide a manual handling structure integral to said first or second polymeric substrate layer.