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Method for detection of Pseudomonas aeruginosa using polymerase chain reaction

  • US 6,048,688 A
  • Filed: 11/12/1996
  • Issued: 04/11/2000
  • Est. Priority Date: 11/12/1996
  • Status: Expired due to Fees
First Claim
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1. A method for detecting the presence of Pseudomonas aeruginosa in a fluid sample using a PCR amplification process to amplify a segment of the P. aeruginosa exotoxin A gene sequence, said method comprising treating strands of nucleotide fragments with a first and a second oligonucleotide primer that hybridize to a region of said nucleotide fragments wherein said segment has from 400 to about 600 base pairs, wherein said first primer has the sequence 5'"'"'-ACA ACG CCC TCA GCA TCA CCA-3'"'"' (SEQ ID NO:

  • 4) and said second primer has the sequence 5'"'"'-CGG GTC GAG CAG GCA CAA C-3'"'"' (SEQ ID NO;

    5), amplifying said segment of the P. aeruginosa exotoxin A gene sequence, and detecting said amplified segment, thereby indicating the presence of P. aeruginosa in said fluid sample.

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