Method and system for performing a binding assay
First Claim
Patent Images
1. A method comprising the steps of:
- separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample;
applying the first subsample to a first binding assay for detecting any of 4m different base sequences having a length of m bases; and
applying the second subsample to a second binding assay for detecting any of 4n different base sequences having a length of n bases.
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Abstract
A method of performing a binding assay which comprises the steps of separating a plurality of sample fragments into a plurality of subsamples and applying each of the plurality of subsamples to a respective one of a plurality of binding assays. A system which performs the aforementioned steps is also disclosed.
220 Citations
22 Claims
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1. A method comprising the steps of:
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separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample; applying the first subsample to a first binding assay for detecting any of 4m different base sequences having a length of m bases; and applying the second subsample to a second binding assay for detecting any of 4n different base sequences having a length of n bases. - View Dependent Claims (2, 3, 4, 5)
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6. A method comprising the steps of:
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providing a plurality of binding assays including a first binding assay and a second binding assay, the first binding assay for detecting any of 4m different base sequences having a length of m bases, the second binding assay for detecting any of 4n different base sequences having a length of n bases; generating a first field within a first channel to separate, within the first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample; generating a second field in a second channel in communication with the first channel to isolate the first subsample within the second channel, wherein the second field is generally perpendicular to the first field; generating a third field in a third channel in communication with the first channel to isolate the second subsample within the third channel, wherein the third field is generally perpendicular to the first field; applying the first subsample isolated in the second channel to the first binding assay; and applying the second subsample isolated in the third channel to the second binding assay. - View Dependent Claims (12, 13, 14)
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7. A method comprising the steps of:
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providing a plurality of binding assays including a first binding assay and a second binding assay, the first binding assay for detecting any of 4m different base sequences having a length of m bases, the second binding assay for detecting any of 4n different base sequences having a length of n bases; separating, within a first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample; isolating the first subsample within a second channel in communication with the first channel, wherein the second channel is generally perpendicular to the first channel; isolating the second subsample within a third channel in communication with the first channel, wherein the third channel is generally perpendicular to the first channel; applying the first subsample isolated in the second channel to the first binding assay; and applying the second subsample isolated in the third channel to the second binding assay. - View Dependent Claims (15, 16, 17)
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8. A method comprising the steps of:
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providing a plurality of binding assays including a first binding assay and a second binding assay; separating, within a first channel, a plurality of unseparated sample fragments into a plurality of subsamples including a first subsample and a second subsample; isolating the first subsample within a second channel connected to the first channel, the second channel having an outlet; isolating the second subsample within a third channel connected to the first channel, the third channel having an outlet; migrating the first subsample to the outlet of the second channel; migrating the second subsample to the outlet of the third channel; providing an applicator having a plurality of transfer elements including a first transfer element and a second transfer element; loading the first subsample from the outlet of the second channel to the first transfer element; loading the second subsample from the outlet of the third channel to the second transfer element; and contacting the applicator to a member which supports the plurality of binding assays to apply the first subsample to the first binding assay and the second subsample to the second binding assay. - View Dependent Claims (9, 10, 11, 18, 19, 20)
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21. A method comprising the steps of:
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separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample; applying the first subsample to a first binding assay for detecting any of 4m different base sequences having a length of m bases; and applying the second subsample to a second binding assay for detecting any of 4n different base sequences having a length of n bases; wherein n is greater than m.
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22. A method comprising the steps of:
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separating a plurality of sample fragments into a plurality of subsamples including a first subsample and a second subsample, the second subsample having sample fragments of generally greater length than the first subsample; applying the first subsample to a first binding assay for detecting any of 4m different base sequences having a length of m bases; and applying the second subsample to a second binding assay for detecting any of 4n different base sequences having a length of n bases; wherein n is greater than m.
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Specification