Optical chemical sensor based on multilayer self-assembled thin film sensors for aquaculture process control

• US 6,051,437 A
• Filed: 05/04/1998
• Issued: 04/18/2000
• Est. Priority Date: 05/04/1998
• Status: Expired due to Fees

• Current Assignee: American Research Corporation Of Virginia
• Original Assignee: American Research Corporation Of Virginia
• Inventors: Lo, K. Peter, Groger, Howard P., Luo, Shufang, Churchill, Russell J.
• Primary Examiner(s): SNAY, JEFFREY R
• Application Number:
  US09/071,775
• Time in Patent Office: 715 Days
• Field of Search: 422/82.05, 422/82.08, 422/82.09, 422/82.11, 436/164, 436/172
• US Class Current: 436/172
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

Optical chemical probes have layers of anionic and cationic polyelectrolytes and one or more dyes incorporated into these layers. The probes are placed into the medium and the dye or dyes react in the presence of the corresponding chemical. Color changes may be observed manually or by a photo detector. A light source may be employed to increase the optical signal received from the probe. Further, a waveguide may be used to trap multiple optical signals. The invention is used for chemical analysis.

Claims

1. An optical chemical probe comprising at least one dye for detecting the presence of chemical analytes, and polymeric film media for binding the at least one dye, wherein the polymeric film media is comprised of consecutive layers of anionic and cationic polyelectrolytes.

2. The probe of claim 1, wherein there are at least 3 and no more than 5 layers of each of the anionic and cationic polyelectrolytes.

3. The probe of claim 1, wherein the at least one dye is a dye having metachromasy property.

4. The probe of claim 3, wherein the dye is a triphenylmethane dye.

5. The probe of claim 1, wherein the at least one dye is diprotonated in acid media selected from a group consisting of bromocresol purple, bromophenol blue, thymol blue or bromothymol blue.

6. The probe of claim 1, wherein the at least one dye bonds to the layers of anionic and cationic polyelectrolytes through hydrogen bonding, charge interaction or other non-covalent methods.

7. The probe of claim 1, further comprising a thin Teflon protective film for coating the sensor.

8. A chemical sensor apparatus comprising at least one dye for detecting the presence of chemical analytes, polymeric film media for binding the at least one dye, a light source for generating optical signals corresponding to changes in the optical absorption spectrum and fluorescence of the dye, and a detector positioned near the polymeric film media for sensing the optical signals, wherein the polymeric film media is comprised of consecutive layers of anionic and cationic polyelectrolytes.

9. The apparatus of claim 8, wherein there are at least 3 and no more than 5 layers of anionic and cationic polyelectrolytes.

10. The apparatus of claim 8, wherein the at least one dye is a dye having metachromasy property.

11. The apparatus of claim 10, wherein the at least one dye is a triphenylmethane dye.

12. The apparatus of claim 8, wherein the at least one dye is diprotonated in acid media selected from a group consisting of bromocresol purple, bromophenol blue, thymol blue or bromothymol blue.

13. The apparatus of claim 8, wherein the at least one dye bonds to the polymeric film media through hydrogen bonding, charge interaction or other non-covalent methods.

14. The apparatus of claim 8, wherein changes in the optical absorption are detected using a standard UV-visible spectrophotometer.

15. The apparatus of claim 8, further comprising a thin Teflon protective film for coating the sensor.

16. The apparatus of claim 8, further comprising a red light source.

17. The apparatus of claim 8, wherein the light source is a modulated light source directed toward the polymeric film media for producing a modulated light signal and for generating optical signals corresponding to changes in the optical absorption spectrum.

18. The apparatus of claim 8, further comprising an electronic light modulator connected to the light source for modulating the light source and a signal processor connected to the light modulator and to the detector for sequentially modulating the light source, for collecting an electronic signal from the detector for providing improved signal-to-noise ratio, for conditioning electronic signals, and for identifying and measuring concentration of an analyte.

19. The apparatus of claim 8, wherein changes in fluorescence are measured using a photodetector equipped with a long-wavelength-pass filter or a bandpass filter.

20. A chemical sensor apparatus comprising an optical waveguide, at least one dye for detecting the presence of chemical analytes;

polymeric film media positioned on the waveguide for binding the at least one dye, a light source for generating optical signals corresponding to changes in the optical absorption spectrum of the dye, and a detector positioned near the waveguide for sensing the optical signals, wherein the waveguide has at least two regions of differing polymeric media with different dyes bound to the polymeric media and wherein the polymeric film media is comprised of consecutive layers of anionic and cationic polyelectrolytes.

21. The apparatus of claim 20, wherein the detector further comprises multiple photodetectors equal in number to a number of the regions, and wherein each photodetector receives and evaluates an optical signal from only one region.

22. A method for identifying concentration of a chemical analyte comprising incorporating at least one dye in consecutive layers of anionic and cationic polyelectrolytes;

placing the layers and dyes into the media to be analyzed; and

detecting changes in the optical absorption of the layers and dyes.

23. The method of claim 22, further comprising using digital Fourier transform techniques to filter extraneous noise sources from the chemical sensor.

24. A method for identifying concentration of a chemical analyte in either gaseous or liquid phase comprising incorporating at least one dye in consecutive layers of anionic and cationic polyelectrolytes;

placing the layers and dyes into the media to be analyzed;

using a light source to create optical signals corresponding to changes in the optical absorption spectrum;

trapping the optical signals;

detecting the optical signals;

collecting the optical signals; and

converting the optical signals to electronic signals.