Figwort mosaic virus promoter and uses

• US 6,051,753 A
• Filed: 12/04/1992
• Issued: 04/18/2000
• Est. Priority Date: 09/07/1989
• Status: Expired due to Term

• Current Assignee: Monsanto Company (Bayer AG)
• Original Assignee: Calgene Incorporated (Bayer AG)
• Inventors: Comai, Luca, Daubert, Stephen Daniel, Sanger, Margaret P.
• Primary Examiner(s): Fox, David T.
• Application Number:
  US07/985,742
• Time in Patent Office: 2,692 Days
• Field of Search: 435/172.3, 435/320.1, 435/240.1, 435/468, 435/419, 800/205, 800/278, 800/286, 800/298, 935/25, 536/24.1, 536/24.5, 536/23.72
• US Class Current: 800/278
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

This invention relates to a novel plant promoter derived from the figwort mosaic virus and methods of use of same.

Claims

1. A recombinant DNA construct capable of transcription in a plant cell comprising in the 5'"'"' to 3'"'"' direction of transcription as operably joined components, a figwort mosaic virus 34S promoter, a DNA sequence of interest heterologous to said promoter and a transcript termination region functional in a plant cell wherein said figwort mosaic virus 34S promoter is at least a quarter length promoter.

2. The recombinant DNA construct of claim 1 wherein said transcript termination region is from the 3'"'"' flanking region of the mannopine synthase gene.

3. The recombinant DNA construct of claim 1 wherein said figwort mosaic virus 34S promoter comprises a TATA box having the sequence TATTTAA.

4. The recombinant DNA construct of claim 1 wherein said figwort mosaic virus 34S promoter comprises at least 196 bp 5'"'"' of the TATTTAA at nucleotides 894 to 900 of FIG. 4.

5. The recombinant DNA construct of claim 1 wherein said figwort mosaic virus 34S promoter comprises at least 362 bp 5'"'"' of the TATTTAA at nucleotides 894 to 900 of FIG. 4.

6. The recombinant DNA construct of claim 1 wherein said figwort mosaic virus promoter comprises at least 892 bp 5'"'"' of the TATTTAA at nucleotides 894 to 900 of FIG. 4.

7. The recombinant DNA construct of claim 1 wherein said DNA sequence of interest is a structural gene.

8. The recombinant DNA construct of claim 1 wherein said DNA sequence of interest is an anti-sense DNA sequence.

9. A DNA cassette for plant genetic engineering applications, wherein said DNA cassette comprises a recombinant DNA construct of claim 1, and a second recombinant DNA construct comprising as operably joined components in the 5'"'"' to 3'"'"' direction of transcriptions, a CaMV 35S promoter, a DNA sequence of interest and a transcript termination region functional in a plant cell.

10. The DNA cassette of claim 9 wherein said DNA sequence of interest joined to said CaMV 35S promoter is different from said DNA sequence of interest joined to said figwort mosaic virus promoter.

11. The recombinant DNA construct of claim 1 further comprising a 5'"'"' untranslated leader sequence.

12. The recombinant DNA construct of claim 11, wherein said 5'"'"' untranslated sequence is from a figwort mosaic virus 34S promoter.

13. A plant cell comprising a recombinant DNA construct of any one of claims 1, 2-8, 11 and 12 or a DNA cassette of any one of claims 9 and 10.

14. A plant comprising a recombinant DNA construct of any one of claims 1, 2-8, 11 and 12 or a DNA cassette of and one of claims 9 and 10.

15. The recombinant DNA construct of claim 1 wherein said figwort mosaic virus 34S promoter is selected from the group consisting of a quarter length promoter, a half length promoter, and a full length promoter.