Acrylic microchannels and their use in electrophoretic applications
First Claim
1. An enclosed microchannel device for use in a method where charged entities are moved through a network of interconnected microchannels under the influence of applied electric fields, the said device comprising a moldable polymeric base member, cover member,. and means for bonding together the base and cover members;
- said base member having at least one substantially planar acrylic surface and a network of interconnected microchannels having cross-sectional dimensions ranging from about 1 μ
m to 200 μ
m provided in said acrylic surface, and adapted to receive charged entities for movement under the influence of said applied electric fields;
said cover member comprising a substantially planar and optically-transparent thin polymeric cover film approximately 1 to 2 mils thick having at least one substantially planar surface;
said base and cover members bonded together by the steps of applying an optically transparent bonding material to at least one of said planar surfaces, partially curing the bonding material, apposing said planar surfaces of said cover member and said base member, and curing said bonding material to form a stable and optically transparent interface between said planar surfaces.
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Accused Products
Abstract
Microchannels having at least an acrylic inner surface and methods of their use in electrophoretic applications are provided. The subject microchannels may be in the form of a variety of configurations suitable for holding an electrophoretic medium. The subject microchannels give rise to substantially reduced EOF and/or adsorption as compared to fused silica under conditions of electrophoresis and find use in a variety of electrophoretic applications in which charged entities are moved through a medium under the influence of the an applied electric field.
411 Citations
7 Claims
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1. An enclosed microchannel device for use in a method where charged entities are moved through a network of interconnected microchannels under the influence of applied electric fields, the said device comprising a moldable polymeric base member, cover member,. and means for bonding together the base and cover members;
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said base member having at least one substantially planar acrylic surface and a network of interconnected microchannels having cross-sectional dimensions ranging from about 1 μ
m to 200 μ
m provided in said acrylic surface, and adapted to receive charged entities for movement under the influence of said applied electric fields;said cover member comprising a substantially planar and optically-transparent thin polymeric cover film approximately 1 to 2 mils thick having at least one substantially planar surface; said base and cover members bonded together by the steps of applying an optically transparent bonding material to at least one of said planar surfaces, partially curing the bonding material, apposing said planar surfaces of said cover member and said base member, and curing said bonding material to form a stable and optically transparent interface between said planar surfaces. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification