Apparatus for multi-well microscale synthesis
First Claim
1. An apparatus for multiple, simultaneous synthesis, comprising:
- a synthesis block, said synthesis block including a plurality of reaction wells integrally formed within said block and arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery;
a first sheet gasket sealable upon the inlet portions;
a second sheet gasket sealable upon the outlet spouts;
an upper cover for pressured engagement with said first sheet gasket;
a lower cover for pressured engagement with said second sheet gasket;
attachment means for releasably and independently attaching each one of said upper and lower covers to said periphery, said attachment means including said synthesis block having at least one protruding structure located on the periphery for attachment of said upper and lower covers; and
said attachment means further includes a reinforcement assembly having an upper collar and a lower collar for opposable sandwiching of the at least one protruding structure, each of said upper and lower covers attached to one of said upper and lower collars.
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Accused Products
Abstract
A multi-well synthesis and filtration apparatus (10) dimensionally arrayed in a standard multi-well format for performing multiple chemical reactions includes a deep-well synthesis block (12) with flowthrough wells (24) fitted with filter disks (48), an upper cover (14), and a lower cover (16). First and second sheet gaskets (18 and 20) seal inlet portions (38) and outlet spouts (40) of the wells (24). An optional skirt (26) provides a site for the independently releasable attachment of the upper and lower covers (14 and 16) to the block (12) while maintaining the seal on the unreleased cover. An alternative synthesis apparatus (310) additionally includes a reinforcement assembly (372) to improve the clamping action of the covers (14 and 16) upon the synthesis block (12). A rotating carrier apparatus (200) and a modified oven assembly (202) are provided to enable agitation and heating of multiple synthesis blocks (12) simultaneously.
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Citations
37 Claims
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1. An apparatus for multiple, simultaneous synthesis, comprising:
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a synthesis block, said synthesis block including a plurality of reaction wells integrally formed within said block and arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery; a first sheet gasket sealable upon the inlet portions; a second sheet gasket sealable upon the outlet spouts; an upper cover for pressured engagement with said first sheet gasket; a lower cover for pressured engagement with said second sheet gasket; attachment means for releasably and independently attaching each one of said upper and lower covers to said periphery, said attachment means including said synthesis block having at least one protruding structure located on the periphery for attachment of said upper and lower covers; and said attachment means further includes a reinforcement assembly having an upper collar and a lower collar for opposable sandwiching of the at least one protruding structure, each of said upper and lower covers attached to one of said upper and lower collars. - View Dependent Claims (2, 3, 4, 5, 6, 7, 9, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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8. An apparatus for multiple, simultaneous synthesis, comprising:
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a synthesis block, said synthesis block including a plurality of reaction wells integrally formed within said block and arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery; a first sheet gasket sealable upon the inlet portions; a second sheet gasket sealable upon the outlet spouts; an upper cover for pressured engagement with said first sheet gasket; a lower cover for pressured engagement with said second sheet gasket; and attachment means for releasably and independently attaching each one of said upper and lower covers to said periphery, said attachment means including said synthesis block having at least one protruding structure located on the periphery for attachment of said upper and lower covers; and a manifold apparatus and said synthesis block further having a block body lower surface, the manifold apparatus including a base, a removable lid, a first gasket, a second gasket, and means for securing the lid to the base, the base having an open chamber sized to receive a collection plate, a top surface, and an outlet for applying a reduced pressure to the chamber, the lid having an open area bounded by a shelf, the first and second gaskets having a generally rectangular band shape, the first gasket interposable between the base top surface and the lid, the second gasket interposable between the shelf and the block body lower surface.
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11. An apparatus for multiple, simultaneous synthesis, comprising:
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a synthesis block, said synthesis block including a plurality of wells arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery and upper and lower synthesis block surfaces; a first sheet gasket sealable upon the inlet portions; a second sheet gasket sealable upon the outlet spouts; an upper cover for pressured engagement with said first sheet gasket; a lower cover for pressured engagement with said second sheet gasket; a first collar and a second collar, said first and second collars opposably located about the periphery; first attachment means for removably attaching the collars about the periphery; second attachment means for releasably and independently attaching each of said upper and lower covers to one of said first and second collars. - View Dependent Claims (10, 12, 13, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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35. An apparatus for multiple, simultaneous synthesis, comprising:
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a synthesis block, said synthesis block including a plurality of reaction wells integrally formed within said block and arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery; a first sheet gasket sealable upon the inlet portions; a second sheet gasket sealable upon the outlet spouts; an upper cover for pressured engagement with said first sheet gasket; a lower cover for pressured engagement with said second sheet gasket; attachment means for releasably and independently attaching each one of said upper and lower covers to said periphery, said attachment means including said synthesis block having at least one protruding structure located on the periphery for attachment of said upper and lower covers; and said outlet spouts have a tapered Luer-tip shape.
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36. An apparatus for multiple, simultaneous synthesis, comprising:
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a synthesis block, said synthesis block including a plurality of reaction wells integrally formed within said block and arranged in ordered array, each well having an inlet portion and an outlet spout, said synthesis block further having a periphery; a first sheet gasket sealable upon the inlet portions; a second sheet gasket sealable upon the outlet spouts; an upper cover for pressured engagement with said first sheet gasket; a lower cover for pressured engagement with said second sheet gasket; attachment means for releasably and independently attaching each one of said upper and lower covers to said periphery, said attachment means including said synthesis block having at least one protruding structure located on the periphery for attachment of said upper and lower covers; said synthesis block has block body of a generally rectangular shape, said block body having an upper surface, a lower surface, and side surfaces; each of said plurality of wells of said synthesis block including a tube bore, each of said tube bores extending between said upper and lower surfaces; said outlet spouts depending from said lower surface, each outlet spout in fluid communication with a said tube bore; said inlet portions extending upwardly from the upper surface, each inlet portion in axial alignment with a said tube bore; said tube bore have a cross-sectional shape selected from the group of shapes consisting of generally rectangular and oval, each outlet spout axially offset with respect to each tube bore to provide alignment of said outlet spouts with the odd-numbered columns of wells of a multi-well collection plate in a first orientation of said block, and alignment with the even-numbered columns of wells of the collection plate in a second orientation in which said block is turned 180 degrees from the first orientation. - View Dependent Claims (37)
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Specification