Oligonucleotide arrays

• US 6,057,100 A
• Filed: 06/06/1997
• Issued: 05/02/2000
• Est. Priority Date: 06/07/1996
• Status: Expired due to Fees

• Current Assignee: PDL BioPharma, Inc.
• Original Assignee: EOS Biotechnology, Inc. (PDL BioPharma, Inc.)
• Inventors: Heyneker, Herbert L.
• Primary Examiner(s): Campbell, Eggerton A.
• Application Number:
  US08/870,514
• Time in Patent Office: 1,061 Days
• Field of Search: 435/6, 435/91.2, 935/77, 935/78, 536/2
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00515 in the shape of strings
• CPC Class Codes: B01J 2219/00518 in the shape of tapes
• CPC Class Codes: B01J 2219/00538 in the shape of cylinders
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/0063 Other, e.g. van der Waals forces, hydrogen bonding
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00657 One-dimensional arrays
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

The invention relates to novel oligonucleotide arrays.

Claims

1. An oligonucleotide array comprising a solid support comprising a plurality of different oligonucleotide pools, each oligonucleotide pool arranged in a distinct linear row to form an immobilized oligonucleotide stripe, wherein the length of each stripe is greater than its width.

2. An array according to claim 1 wherein said pools are covalently attached.

3. An array according to claim 1 wherein said solid support is plastic.

4. An array according to claim 1 wherein each of said rows are parallel to the other rows.

5. An oligonucleotide array according to claim 1 wherein said solid support is a single support surface.

6. An oligonucleotide array according to claim 1 wherein said stripes are laid down at a density of at least one per millimeter.

7. An oligonucleotide array according to claim 1 wherein said stripes are laid down at a density of at least two per millimeter.

8. An oligonucleotide array according to claim 1 wherein said stripes are laid down at a density of at least three per millimeter.

9. An oligonucleotide array according to claim 1 wherein said solid support comprises a plurality of support surfaces.

10. An oligonucleotide array according to claim 9 wherein each of said oligonucleotide pools is attached to a different support surface.

11. An array according to claim 10, wherein said array is in woven form.

12. An array according to claim 10, wherein said array is in alignment form.

13. An array according to claim 1, wherein said array is in circular form.

14. A composite array comprising at least one strip of a first array according to claim 1 and at least one strip of a second array according to claim 1, wherein at least one covalently attached oligonucleotide of the second array is not present in said first array, wherein said strips are adjacent.

15. A method of making an oligonucleotide array according to claim 5 or 9 comprising:

a) activating said support surface; and

b) adding said oligonucleotide pools to said activated support surface to form said oligonucleotide stripes.

16. A method according to claim 15 wherein said adding is done by drawing.

17. A method according to claim 15 wherein said adding is done by piezoelectric pump.

18. A method of detecting the presence or absence of a target sequence in a sample comprising

a) contacting said sample with an array according to claim 1 under conditions that permit hybridization of said target sequence, if present, to an oligonucleotide covalently attached to said array;

and

detecting the presence or absence of said target sequence.

19. A kit comprising an array according to claim 1 and at least one reagent.