Nucleic acids encoding ligands for HEK4 receptors

• US 6,057,124 A
• Filed: 01/27/1995
• Issued: 05/02/2000
• Est. Priority Date: 01/27/1995
• Status: Expired due to Fees

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Fox, Gary M., Bartley, Timothy D.
• Primary Examiner(s): Ulm, John
• Assistant Examiner(s): MERTZ, PREMA MARIA
• Application Number:
  US08/379,802
• Time in Patent Office: 1,922 Days
• Field of Search: 435/69.1, 435/70.1, 435/70.3, 435/71.1, 435/71.2, 435/240.1, 435/240.2, 435/320.1, 435/252.3, 435/23.1, 435/252.33, 435/471, 935/22, 935/27, 935/29, 935/31, 935/52, 935/55, 935/56, 935/57, 935/66, 935/70, 935/72, 935/73, 536/23.5, 536/24.3
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/52 Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 2319/00 Fusion polypeptide

Abstract

Polypeptides which bind to one or more EPH-like receptors, particularly the HEK4 receptor, are described. The polypeptides are designated HEK4 binding proteins. Nucleic acids encoding HEK4 binding proteins, and expression vectors, host cells and processes for the production of the polypeptides are also described. The polypeptides are useful for modulating the growth and/or differentiation of a variety of tissues, including those from liver, kidney, lung, skin, digestive tract and nervous system and may be used to regenerate damaged or depleted tissue and to treat cancer or nervous system disorders.

Claims

1. An isolated nucleic acid encoding a polypeptide which binds to at least a HEK4 receptor wherein the nucleic acid is selected from the group consisting of:

a) the nucleic acid having the sequence shown in SEQ ID NO;

1 and its complementary strand;

b) nucleic acids which hybridize under conditions of 6×

SSC and 65°

C. and remain hybridized under conditions of 0.2×

SSC and 65°

C. with the coding region of SEQ ID NO;

1; and

c) nucleic acids which are degenerate to nucleic acids set forth in (a) and (b).

2. The nucleic acid of claim 1 which is cDNA, genomic DNA or synthetic DNA.

3. The nucleic acid of claim 1 which comprises one or more codons preferred for expression in Escherichia coli.

4. An expression vector comprising the nucleic acid of claim 1.

5. A procaryotic or eucaryotic host cell stably transformed or transfected with the vector of claim 4.

6. The host cell of claim 5 which is a CHO cell.

7. The host cell of claim 5 which is Escherichia coli.

8. A process for the production of a polypeptide which binds at least a HEK4 receptor comprising growing under suitable nutrient conditions a procaryotic or eukaryotic host cell transformed or transfected with the nucleic acid of claim 1 and isolating the polypeptide product of the expression of the nucleic acid.