Tissue-equivalent rods containing aligned collagen fibrils and schwann cells

• US 6,057,137 A
• Filed: 10/04/1996
• Issued: 05/02/2000
• Est. Priority Date: 10/06/1994
• Status: Expired due to Term

• Current Assignee: University of Minnesota
• Original Assignee: Regents of The University of Minnesota (University of Minnesota)
• Inventors: Girton, Timothy Sanuel, Mooradian, Daniel L., Tranquillo, Robert T., Guido, Stefano
• Primary Examiner(s): Saucier, Sandra E.
• Application Number:
  US08/725,916
• Time in Patent Office: 1,306 Days
• Field of Search: 424/422, 424/426, 623/12, 435/382, 435/174
• US Class Current: 435/174
• CPC Class Codes: A61B 17/11 for performing anastomosis; Buttons for anastomosis
• CPC Class Codes: A61B 17/1128 of nerves
• CPC Class Codes: A61B 2017/00004 (bio)absorbable, (bio)resorbable or resorptive
• CPC Class Codes: A61F 2/06 Blood vessels
• CPC Class Codes: A61L 2430/32 for nerve reconstruction
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: A61L 31/04 Macromolecular materials
• CPC Class Codes: A61L 31/044 Collagen

Abstract

Tissue-equivalent and biopolymer tubes and rods include fibrils which are oriented (aligned) by a magnetic field. These oriented fibrils provide enhanced mechanical and cell guidance properties to the tissue-equivalent and biopolymer tubes. One such tissue-equivalent tube includes a body of collagen gel with mammalian tissue cells interspersed therein. The collagen fibrils are circumferentially oriented within the tubular body by a magnetic field, thereby inducing circumferential orientation of the cells. One such biopolymer rod includes collagen fibrils longitudinally oriented along the rod axis by a magnetic field that guides invasion of cells. Methods of making magnetically oriented tissue-equivalent and biopolymer tubes and rods are also disclosed.

Claims

1. A method of forming a tissue-equivalent rod comprising:

a. providing a rod-shaped mold extending in the longitudinal and transverse directions, the mold including a cavity, the mold including an axis extending in the longitudinal direction;

b. filling the cavity of the mold with a solution comprising collagen and Schwann cells; and

c. placing the filled mold into a magnetic field, such that the longitudinal axis of the mold is substantially perpendicular to the direction of the magnetic field, whereby the fibrils forming in the collagen align and extend in the longitudinal direction.

2. The method of claim 1, wherein the rod-shaped mold is cylindrical.

3. The method of claim 1, wherein the collagen is monomeric Type I collagen.

4. The method of claim 1, wherein the filling step involves injecting the solution of collagen and cells.

5. The method of claim 1, wherein the magnetic field is a static magnetic field.

6. The method of claim 1, wherein the rod-shaped mold includes a biodegradable or bioresorbable polymer outer casing.

7. The method of claim 1, additionally comprising:

d. removing the filled mold from the magnetic field; and

e. removing the mold from the formed tissue-equivalent rod.

8. A tissue-equivalent rod, the rod including a cylindrical body including a longitudinal axis, the body extending along the longitudinal axis and comprising collagen and Schwann cells interspersed within the collagen, the collagen formed into fibrils, the fibrils aligned in a direction substantially parallel to the longitudinal axis within the body.

9. The tissue-equivalent rod of claim 8, wherein the collagen is monomeric Type I collagen.

10. The tissue-equivalent rod of claim 8, additionally comprising an outer casing including a biodegradable or bioresorbable polymer cylinder.