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Surrogate tolerogenesis for the development of tolerance to xenografts

  • US 6,060,049 A
  • Filed: 06/06/1995
  • Issued: 05/09/2000
  • Est. Priority Date: 05/24/1993
  • Status: Expired due to Term
First Claim
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1. A method for xenograft transplant of an organ to a recipient mammal from a donor mammal, wherein said recipient has increased immuno tolerance to donor tissue, comprising the steps of:

  • a) collecting a first cell population from the recipient, said first cell population containing lymphocytic progenitor cells, wherein at least a portion of cells that are specifically cytotoxic to tissue from a surrogate animal are removed from said first cell population;

    b) administering said first cell population to said surrogate, said surrogate being in a state of immune deficiency;

    c) developing in said surrogate a state of immune competence;

    d) collecting from said immune competent surrogate a second population of cells, said second cell population containing immunocompetent cells, said immunocompetent cells specifically suppressing immune response of said recipient to tissue of said surrogate;

    e) infusing said second population of cells into said recipient;

    f) excising an organ from a donor mammal wherein the donor mammal is selected from the group consisting of the same surrogate mammal, the same inbred strain as the surrogate mammal and a littermate of the surrogate mammal; and

    g) transplanting said organ into said xenograft recipient.

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