Sensors for sugars and other metal binding analytes
First Claim
1. A system for measuring the concentration of target molecules in solution, comprising:
- (a) a sensing material comprising metal complexes having at least two coordination sites available for binding at least one of the target molecules selected from the group consisting of a sugar, a monosaccharide, a disaccharide, a trisaccharide, an oligosaccharide, an amino sugar, a sugar-amino acid, an amino acid, an amino alcohol, an α
-hydroxycarboxylic acid, a sugar-peptide, a glycoprotein, glycerol, dopamine, a catechol, ascorbic acid, a polyol, a diol, ethyleneglycol, sialic acid, carbonate, phosphate, sulfate, CO, NO, 3-hydroxy-L-tyrosine, and a molecule having multiple hydroxyl groups, the metal complexes releasing protons or hydroxide ions upon binding of the target molecules; and
(b) a detector for measuring the protons or hydroxide ions released from the sensing material and for providing a measurement of the concentration of the target molecules in the solution,wherein the detector is selected from the group consisting of a pH electrode, a field effect transistor, a light addressable potentiometric sensor, and a solution conductivity measuring device, orwherein the detector measures optical or electrical characteristics of the sample and the system further comprises a chemical probe whose optical or electrical properties are sensitive to pH.
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Accused Products
Abstract
Sensors (20, 50, 70) for use in detecting the presence of sugars and other analytes (target molecules). The sensor is composed of a metal complex that binds to the target molecule and releases a proton or includes an exchangable ligand which is exchanged for the target molecule during the binding interaction between the metal complex and the target molecule. The result of the binding interaction is the release of a proton, hydroxide ion or ligand species generated during the ligand exchange. Measurement of the release of proton, hydroxide ion or other ligand species from the sensor (20, 50, 70) provides an indirect indication of target molecule concentration. The metal complexes may be attached to support structures (10, 12) to provide both anchoring and positioning of the metal ions to increase selectivity of sugar/metal complex interactions. Detection systems in which pH is used as an indication of proton or hydroxide release are disclosed, as are detection systems in which Cl- release is used. Methods for monitoring the concentrations of sugars and related molecules using the metal based sensors (20, 50, 70) are also disclosed.
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Citations
32 Claims
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1. A system for measuring the concentration of target molecules in solution, comprising:
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(a) a sensing material comprising metal complexes having at least two coordination sites available for binding at least one of the target molecules selected from the group consisting of a sugar, a monosaccharide, a disaccharide, a trisaccharide, an oligosaccharide, an amino sugar, a sugar-amino acid, an amino acid, an amino alcohol, an α
-hydroxycarboxylic acid, a sugar-peptide, a glycoprotein, glycerol, dopamine, a catechol, ascorbic acid, a polyol, a diol, ethyleneglycol, sialic acid, carbonate, phosphate, sulfate, CO, NO, 3-hydroxy-L-tyrosine, and a molecule having multiple hydroxyl groups, the metal complexes releasing protons or hydroxide ions upon binding of the target molecules; and(b) a detector for measuring the protons or hydroxide ions released from the sensing material and for providing a measurement of the concentration of the target molecules in the solution, wherein the detector is selected from the group consisting of a pH electrode, a field effect transistor, a light addressable potentiometric sensor, and a solution conductivity measuring device, or wherein the detector measures optical or electrical characteristics of the sample and the system further comprises a chemical probe whose optical or electrical properties are sensitive to pH. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10)
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2. A system for measuring the concentration of target molecules in solution, comprising:
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(a) a sensing material comprising metal complexes having the formula
space="preserve" listing-type="equation">L.sub.1 --M--L.sub.2wherein M is a metal ion;
L1 is a nitrogen based polydentate ligand; and
L2 is an exchangeable ligand;the metal complexes having at least two coordination sites available for binding at least one of the target molecules selected from the group consisting of a sugar, a monosaccharide, a disaccharide, a trisaccharide, an oligosaccharide, an amino sugar, a sugar-amino acid, an amino acid, an amino alcohol, an α
-hydroxycarboxylic acid, a sugar-peptide, a glycoprotein, glycerol, dopamine, a catechol, ascorbic acid, a polyol, a diol, ethyleneglycol, sialic acid, carbonate, phosphate, sulfate, CO, NO, 3-hydroxy-L-tyrosine, and a molecule having multiple hydroxyl groups, the metal complexes releasing exchangeable ligands upon binding of the target molecules; and(b) a detector for measuring the exchangeable ligands released from the sensing material and for providing a measurement of the concentration of the target molecules in the solution. - View Dependent Claims (11, 12, 13, 14, 15, 16)
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17. A method for measuring the concentration of target molecules in a solution, comprising the steps of:
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(a) placing the solution in contact with a sensing material comprising metal complexes wherein each metal complex has the property of; (i) holding a metal ion tightly in the complex; (ii) allowing at least two coordination sites to be or become available for binding to one of the target molecules; (iii) binding the target molecule in solution, wherein the target molecule is selected from the group consisting of a sugar, a monosaccharide, a disaccharide, a trisaccharide, an oligosaccharide, an amino sugar, a sugar-amino acid, an amino acid, an amino alcohol, an α
-hydroxycarboxlic acid, a sugar-peptide, a glycoprotein, glycerol, dopamine, a catechol, ascorbic acid, a polyol, a diol, ethyleneglycol, sialic acid, carbonate, phosphate, sulfate, CO, NO, 3-hydroxy-L-tyrosine, and a molecule with multiple hydroxyl groups; and(iv) upon binding of the metal complex with the target molecules, releasing a detectable entity or entities selected from the group consisting of protons, hydroxide ions and exchangeable ligands wherein the number of released detectable entities is proportional to the concentration of the target molecules in the solution; (b) binding the target molecules in the solution to the metal complexes; (c) releasing the detectable entities into the solution; and (d) measuring the amount of detectable entities released from the sensing material to provide a measurement of the concentration of target molecules in the solution. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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24. The method according to claim 23 wherein the L1 -M portion of said metal complex is selected from the group consisting of copper(II)-triazacyclononane(Cu(TACN)), copper(II)-1,4-dialkyltriazaclononane, copper(II)-1,4-dimethyl triazacyclononane (Cu(1,4-dimethyl-TACN)), copper(II)-ethylenediamine (Cu(En)), copper(II)-propylenediamine (Cu(II)-PDN)), copper(II)-iminodiacetate (Cu(IDA)), copper(II)-diethylenetriamine(Cu(Dien), copper(II) 1-oxa-4,7-diazacyclononane, copper(II) 1-thia-4,7-diazacyclononane and derivatives thereof.
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25. The method according to claim 23 wherein the L1 -M portion of said metal complex is copper(II)-triazacyclononane (Cu(TACN)) and derivatives thereof.
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26. The method according to claim 23 wherein L2 is selected from the group consisting of ionic species, amines, amino acids, phenol, carboxylic acids, amino alcohols, diols, and derivatives thereof.
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27. The method according to claim 26 wherein L2 is measured by fluorescence, chemiluminescence, phosphorescence, colorimetrically or electrochemically.
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28. The method according to claim 17 wherein the target molecule is glucose.
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29. The method according to claim 17 wherein the metal complexes are attached to a support structure.
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30. The method according to claim 29 wherein the support structure comprises an organic or inorganic polymer.
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31. The method according to claim 24 wherein the support structure is an optically transparent surface selected from the group consisting of quartz, glass or a fiber optic cable.
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32. The method according to claim 17 wherein each of the metal complexes contains a polymerizable moiety preorganized with a template molecule to form a templated metal complex, the tenplated metal complex being embedded by copolymerization with at least one monomer, crosslinking agent or a combination thereof into an imprinted polymeric matrix, wherein the template molecule is the target molecule or an analog of the target molecule.
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Specification