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Optical measurement of blood hematocrit incorporating a self-calibration algorithm

  • US 6,064,474 A
  • Filed: 02/06/1998
  • Issued: 05/16/2000
  • Est. Priority Date: 02/06/1998
  • Status: Expired due to Fees
First Claim
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1. A method for determining hematocrit of a blood sample, comprising the steps of:

  • measuring absorbance of said blood sample at a first isobestic wavelength;

    measuring absorbance of said blood sample at a second isobestic wavelength; and

    determining hematocrit of said blood sample in accordance with the algorithm;

    
    
    space="preserve" listing-type="equation">Hct=(T.sub.i,1 -R*T.sub.i,2)/(K.sub.1 -R*K.sub.2),wherein;

    Ti is total absorbance at an isobestic wavelength;

    K is a constant that includes a molar extinction coefficient Hb at that wavelength, a red blood cell scattering factor, and path length;

    Hct is hematocrit; and

    R is the ratio of light scattering from a plasma component measured at two wavelengths.

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