Multi-array, multi-specific electrochemiluminescence testing
First Claim
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1. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and a support comprising an electrode capable of inducing electrochemiluminescence and a counterelectrode, said electrode having immobilized on a surface thereof a binding reagent capable of binding said analyte or a binding partner of said analyte.
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Abstract
Materials and methods are provided for producing patterned multi-array, multi-specific surfaces which are electronically excited for use in electrochemiluminescence based tests. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use in flat panel displays and multiply specific testing procedures.
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Citations
119 Claims
- 1. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and a support comprising an electrode capable of inducing electrochemiluminescence and a counterelectrode, said electrode having immobilized on a surface thereof a binding reagent capable of binding said analyte or a binding partner of said analyte.
- 2. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and an electrode capable of inducing electrochemiluminescence, said electrode having immobilized on a surface thereof a binding reagent capable of directly or indirectly binding said analyte or a binding partner of said analyte, wherein said electrode comprises carbon nanotubes, carbon black, carbon nanotubes in a matrix, carbon black in a matrix or combinations thereof.
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7. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and a porous electrode capable of inducing electrochemiluminescence, said electrode having immobilized on a surface thereof a binding reagent capable of binding a component of a binding electrochemiluminescence assay.
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8. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and an electrode capable of inducing electrochemiluminescence and a porous matrix in electrochemical contact with said electrode or capable of being in electrochemical contact with said electrode, said porous matrix containing on a surface thereof a binding reagent capable of binding a component of a binding electrochemiluminescence assay.
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9. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
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(a) an electrode; (b) a support having immobilized thereon a plurality of discrete binding domains each containing a binding reagent capable of binding a component of a binding electrochemiluminescence assay; and (c) a light detector. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 23, 24, 25)
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- 21. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector and an electrode, said electrode having immobilized on a surface thereof a plurality of discrete binding domains, said binding domains being capable of binding a component of a binding electrochemiluminescence assay.
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28. A kit for use in performance of a plurality of electrochemiluminescence assays for analytes of interest comprising:
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(a) an article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and at least two binding domains having different binding specificities for analytes of interest in an electrochemiluminescence assay; and (b) a reagent comprising an electrochemiluminescent label. - View Dependent Claims (29, 103, 104, 105, 107, 109, 110)
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30. A kit for use in performance of a plurality of electrochemiluminescence assays for a plurality of analytes of interest comprising:
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(a) an article comprising a plurality of discrete binding domains on an electrode, said binding domains having a relative spatial organization with respect to one another and at least two binding domains having different binding specificities for analytes of interest in an electrochemiluminescence assay; and (b) a reagent comprising an electrochemiluminescent label. - View Dependent Claims (106, 108)
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31. A system for conducting a plurality of electrochemiluminescence assays comprising:
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(a) a plurality of discrete binding domains specific for a plurality of different analytes; (b) a voltage waveform generator; and (c) a light detector capable of simultaneously detecting light from more than one binding domain.
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32. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
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(a) a support having a plurality of discrete binding domains thereon; (b) an electrode capable of inducing electrochemiluminescence, wherein said plurality of discrete binding domains and said electrode are capable of being brought into proximity; (c) a source of electrical energy for generating electrochemiluminescence; and (d) a light detector. - View Dependent Claims (40, 59, 60, 63, 65, 76, 83, 84, 85, 86)
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33. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
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(a) a support having a plurality of discrete binding domains thereon, wherein said support comprises an electrode for generating electrochemiluminescence; (b) a source of electrical energy for generating electrochemiluminescence; and (c) a light detector.
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34. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
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(a) a cassette, wherein said cassette comprises an electrode and a plurality of discrete binding domains, wherein one or more binding domains contain binding reagents of specificity different from other binding domains; (b) a receptacle for said cassette; (c) a source of electrical energy for generating electrochemiluminescence; and (d) a light detector.
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35. A apparatus for use in the detection of an analyte by electrochemiluminescence comprising:
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(a) a plurality of discrete binding domains immobilized on a support; and (b) a plurality of electrodes capable of inducing electrochemiluminescence, wherein said binding domains are spatially aligned with said plurality of electrodes. - View Dependent Claims (66, 78, 80, 82, 87, 89, 95, 96, 102)
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36. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a plurality of electrodes capable of inducing electrochemiluminescence and a light detector, said electrodes having immobilized on surfaces thereof binding reagents capable of binding components of a binding electrochemiluminescence assay.
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37. An apparatus for use in the detection of an analyte by electrochemiluminescence comprising a light detector, a plurality of electrodes capable of inducing electrochemiluminescence and a plurality of discrete binding domains proximate said electrodes, said plurality of electrodes being capable of inducing electrochemiluminescence from at least one binding domain, each binding domain having an amount of binding reagent capable of binding a component of a binding electrochemiluminescence assay.
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38. An apparatus for generating electrochemiluminescence comprising:
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(a) a support; (b) an electrode on said support having a plurality of discrete binding domains immobilized thereon; (c) a counterelectrode on said support; and (d) a light detector.
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39. An apparatus for generating electrochemiluminesence comprising:
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(a) a support; (b) a plurality of electrodes on said support, said electrodes having binding domains immobilized thereon; (c) a counterelectrode on said support; and (d) a light detector.
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111. A kit for use in performance of a plurality of electrochemiluminescence assays for a plurality of analytes of interest comprising:
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(a) an article comprising a plurality of discrete binding domains on a support, said binding domains having a relative spatial organization with respect to one another and having different binding specificities for a plurality of different analytes of interest in an electrochemiluminescence assay; and (b) a vessel containing a reagent necessary for conducting said assays. - View Dependent Claims (112, 116, 117, 118, 119)
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113. A kit for use in performance of a plurality of electrochemiluminescence assays for a plurality of analytes of interest comprising:
a vessel containing binding components specific for a plurality of different analytes for use in a plurality of electrochemiluminescence assays, wherein at least two of said binding components have specificity for different analytes and being linked to electrochemiluminescent labels having wavelengths of emission that are substantially identical. - View Dependent Claims (114, 115)
Specification