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Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding

  • US 6,066,459 A
  • Filed: 06/19/1998
  • Issued: 05/23/2000
  • Est. Priority Date: 08/18/1993
  • Status: Expired due to Fees
First Claim
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1. A method of hybridization based multicolor chromosome banding comprising the steps of:

  • (a) providing a metaphase chromosome spread of a first species and chromosome paints of a second species, at least some of said chromosome paints being labeled with a different fluorophore or combination of fluorophores;

    (b) hybridizing said chromosome paints with said metaphase chromosome spread;

    (c) using a fluorescence microscope being optically coupled to a multiband collection device for collecting spectral data from said chromosome spread; and

    (d) based on said collected data calculating a color image in which pixels associated with each of said different fluorophores or combination of fluorophores are attributed distinctive colors, thereby a banded color image of said metaphase chromosome spread is obtainable;

    wherein said method is for an application selected from the group consisting of providing a color banding karyotype of embryonic cells, providing a color banding karotype of white blood cells, providing a color banding karyotype of malignant cells, providing a color banding karyotype of cells examined for malignancy, detecting the stage of a malignancy, following the efficiency of an anti-cancer treatment and detecting chromosomal aberrations following exposure to mitogenic agents.

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