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Morphological mutants of filamentous fungi

  • US 6,066,493 A
  • Filed: 03/13/1997
  • Issued: 05/23/2000
  • Est. Priority Date: 01/19/1996
  • Status: Expired due to Term
First Claim
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1. A method for obtaining a mutant cell which produces a heterologous protein, comprising:

  • (a) producing a first population of presumptive mutant cells from an Aspergillus or a Fusarium parent cell;

    (b) identifying from the first population a second population of presumptive mutant cells having a more restricted colonial phenotype or a more extensive hyphal branching than the parent cell; and

    (c) identifying from the second population the mutant cell, comprising a nucleic acid sequence encoding a heterologous polypeptide, which has a radial extension rate which is at least 10% less than the parent cell, has a hyphal growth unit length that is at least 10% less than the parent cell, and has one or more improved properties selected from the group consisting of (i) produces at least about 10% more heterologous polypeptide than the parent cell, (ii) exhibits about 90% or less of the viscosity of the parent cell, and (iii) secretes more heterologous protein than the parent cell, when cultivated under the same conditions; and

    (d) obtaining the mutant cell.

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